类肠膜魏斯氏菌MbWp142冻干菌粉工艺优化及储藏稳定性研究  

Study on process optimization and storage stability of lyophilized powder of Weissella paramesenteroides MbWp142

作　　者：黄芳 吴佳敏 陈锶 洪真真 范铮 黄娟 黄燕燕 HUANG Fang;WU Jiamin;CHEN Si;HONG Zhenzhen;FAN Zheng;HUANG Juan;HUANG Yanyan

机构地区：[1]佛山大学食品科学与工程学院,广东省食品智能制造重点实验室,广东佛山528225 [2]华南理工大学食品科学与工程学院,广东广州510641

出　　处：《饲料研究》2025年第2期134-138,共5页Feed Research

基　　金：国家自然科学基金(项目编号:32302242);广东省科技创新战略专项资金(项目编号:2022B1212010015);广东省基础与应用基础研究基金(项目编号:2022A1515110426);广东省重点研发项目(项目编号:2023B0202040002)。

摘　　要：试验旨在优化类肠膜魏斯氏菌MbWp142冻干菌粉工艺,研究其储藏稳定性。试验确定了MbWp142最佳离心条件,通过单因素和正交试验优化冻干保护剂配方,进一步提高冻干菌粉的存活率,探究冻干菌粉储藏稳定性。结果显示:MbWp142的最佳离心条件为8 000 r/min离心30 min;冻干保护剂配方为葡萄糖添加量6%、细菌学蛋白胨添加量4%、脱脂乳粉添加量3%的条件下,冻干菌粉存活率高达96.67%,比未加冻干保护剂的菌粉存活率提高了79.12%,且菌粉能够在-20℃下储藏28 d以上。研究表明,试验所用工艺明显提升了MbWp142冻干菌粉活力,可为制备饲用微生态制剂提供参考。The aim of the experiment was to optimize the process of freeze-dried bacteria powder of Weissella paramesenteroides MbWp142 and study its storage stability.The optimal centrifugation conditions for MbWp142 were first determined,followed by optimization of the lyophilized protective agent formula through single-factor and orthogonal experiments to enhance the survival rate of the lyophilized powder.The storage stability of the powder was also investigated.The results showed that the optimal centrifugation conditions were 8000 r/min for 30 min.Under the conditions of 6%glucose,4%bacteriological peptone and 3%defatted milk,the survival rate of the lyophilized powder was 96.67%,which was 79.12%higher than that of the non-lyophilized powder,and the powder could be stored at-20℃for more than 28 days.The study indicates that the optimized process significantly enhanced the vitality of MbWp142 lyophilized powder,providing a reference for the preparation of probiotic feed additives.

关 键 词：类肠膜魏斯氏菌 冻干保护剂 菌粉制备 

分 类 号：S816.7[农业科学—饲料科学]