GAOs丰度变化对聚磷生物膜系统的影响——磷富集性能及代谢特征为例  

Effects of GAOs abundance to the systemmatic phosphorus enrichment performance and the metabolic characteristics in phosphorus-accumulating biofilm

作　　者：毕贞 钱萌萌 袁艺娜 王雪玲 宋歌 黄勇[1,2] BI Zhen;QIAN Meng-meng;YUAN Yi-na;WANG Xue-ling;SONG Ge;HUANG Yong(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China;National and Local Joint Engineering Laboratory for Municipal Sewage Resource Utilization Technology,Suzhou University of Science and Technology,Suzhou 215009,China)

机构地区：[1]苏州科技大学环境科学与工程学院,江苏苏州215009 [2]苏州科技大学,城市生活污水资源化利用技术国家地方联合工程实验室,江苏苏州215009

出　　处：《中国环境科学》2025年第3期1601-1611,共11页China Environmental Science

基　　金：国家自然科学基金资助项目(51938010)。

摘　　要：在厌氧/好氧交替运行的序批式反应器中,以氧化沟活性污泥为接种污泥进行聚磷生物膜的富集培养,通过考察生物膜中聚糖微生物(GAOs)丰度变化对系统磷富集性能以及微生物群落代谢特征的影响,解析GAOs在生物膜磷富集系统中的潜在功能.结果表明,在聚磷生物膜的富集培养过程中,GAOs成为优势菌,但并未对生物膜系统的磷去除与富集效果产生不利影响.由于聚磷微生物(PAOs)单个细胞的PHA代谢活性和poly-P代谢活性均显著增强,使得生物膜群落整体以聚磷代谢模式(PAM)为主导.GAOs作为系统中的优势菌属,可能通过EMP代谢途径获取合成PHA所需的还原力(NADH),为PAOs在好氧条件下吸磷提供充足的能量储备,从而激发无机磷转运系统(pst)、poly-P合成代谢基因(ppk)的表达上调,使生物膜系统表现出良好的磷去除与富集能力,且GAOs(Candidatus Competibacter)作为潜在的反硝化功能菌使得生物膜磷富集系统具备同步好氧脱氮能力.In a sequencing batch reactor with alternating anaerobic/aerobic operation,activated sludge from an oxidation ditch was used as the inoculated sludge for culturing poly-phosphate biofilm enrichment.The potential functions of GAOs in the biofilm phosphorus enrichment system were investigated by examining the effects of the changes in the abundance of glycogen-accumulating organisms(GAOs)in the biofilm on the phosphorus enrichment performance and the metabolic characteristics of the microbial community.The results showed that GAOs became the dominant organisms in the enrichment culture of phosphorus-accumulating biofilm,but they did not adversely affect the phosphorus removal and enrichment of the biofilm system.Due to the significant increase of PHA metabolic activity and poly-P metabolic activity in individual cells of phosphorus-accumulating organisms(PAOs),the biofilm community as a whole was dominated by the phosphorus accumulating metabolism(PAM).GAOs as a dominant bacterial genus in the system,might obtain the reducing power(NADH)required for synthesizing PHA through EMP metabolism,which could provide sufficient energy reserve for the absorption of phosphorus by the PAOs in aerobic conditions,and thus stimulate the phosphorus removal and enrichment effect in the biofilm system.The inorganic phosphorus transport system(pst)and poly-P synthesizing genes(ppk)were up-regulated,so that the biofilm system showed good phosphorus removal and enrichment ability,and the GAOs(Candidatus Competibacter),as a potential denitrifying bacterium,could synchronize with the aerobic denitrification in the biofilm phosphorus-enrichment system.

关 键 词：污水磷富集 聚磷生物膜 聚糖菌 Candidatus Competibacter 代谢特征 

分 类 号：X172[环境科学与工程—环境科学]