在线微管反应器光催化氧化衍生-荧光光度法测定叶酸  

Online Photochemical Oxidation Derivatization Combined with Fluorescence Spectrometry for the Determination of Folic Acid Based on Microtubule Reactor

作　　者：张凯龙[1] 郭雨微 陈锦仪 高俊 谭志文[1] 汪财生[1] ZHANG Kailong;GUO Yuwei;CHEN Jinyi;GAO Jun;TAN Zhiwen;WANG Caisheng(College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo 315100,Zhejiang)

机构地区：[1]浙江万里学院生物与环境学院,浙江宁波315100

出　　处：《中国食品学报》2025年第2期433-440,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省基础公益研究计划项目(LGC21B070003);浙江省“生物工程”一流学科科研项目(ZS2021010);“食品质量与安全”浙江省一流专业建设项目(教高厅函﹝2022﹞14号);国家级大学生创新创业训练计划项目(202310876017)。

摘　　要：叶酸是重要的食物来源维生素。为提高叶酸测定效率,本文建立了一种基于微管反应器的在线光催化氧化衍生联合荧光光度法测定叶酸的系统和方法,优化运行参数,考察评价方法并应用于实际样品测定。结果表明,小孔径石英螺旋管微反应器成功腐蚀涂覆纳米二氧化钛催化剂,紫外光照射流动叶酸样品实现在线光催化氧化衍生,最佳衍生条件pH 4.2、流速250μL/min,荧光最大激发波长和发射波长位于280 nm和442 nm,叶酸在质量浓度0.1~2.0 mg/L范围与荧光强度呈线性相关,标准曲线方程F=381.68c+10.53,相关系数达到0.998,检出限(3s/k)为7.2μg/L,运行稳定性相对平均偏差为0.9%~2.4%,对多种常见物质具有抗干扰能力。应用本法测定叶酸片剂含量误差在5%以内,加标回收率在94.7%~102.1%范围,相对平均偏差不超过2.1%。Folic acid is an important food source vitamin.In order to improve the efficiency of folic acid determination,a system and method for the determination of folic acid by on-line photocatalytic oxidation derivatization combined with fluorescence spectrophotometry based on microtubular reactor was developed.System operating parameters were optimized,and the method was investigated and evaluated,then applied to the determination of actual samples.The results showed that nano-titanium dioxide catalyst was successfully corroded and coated in spiral tube quartz microreactor with small inner diameter,and folic acid on-line derivatization was realized by photocatalytic oxidation with ultraviolet light irradiation.The optimal derivatization pH was 4.2,flow rate was 250μL/min,the maximum excitation and emission wave length were 280 nm and 442 nm.Under the optimal conditions.There was a good linear relationship between fluorescence intensity and folic acid concentration in the range of 0.1-2.0 mg/L.The standard curve equation was F=381.68c+10.53,the correlation coefficient and detection limit(3s/k)was 0.998 and 7.2μg/L,respectively.The relative average deviation of operation stability was 0.9%-2.4%,meanwhile,it has excellent anti-interference ability to a variety of common substances.When the method was applied to folic acid tablets,it exhibited a relative error was less than 5%,the recovery rate was between 94.7%to 102.1%,and the relative average deviation was less than 2.1%.

关 键 词：叶酸 荧光光度法 光催化 纳米二氧化钛 在线分析 

分 类 号：O657.3[理学—分析化学] TS207.3[理学—化学]