miR-30a-5p对乳腺癌细胞中HER2基因及TMSG-1蛋白表达的影响  

Effect of miR-30a-5p on HER2 Gene and TMSG-1 Protein Expression in Breast Cancer Cells

作　　者：张待翼 赵丹 王浩宇 徐树明[2] ZHANG Daiyi;ZHAO Dan;WANG Haoyu;XU Shuming(Department of Breast Surgery,Shanxi Maternal and Child Health Hospital,Taiyuan,Shanxi 030013,China;Medical Imaging Center,Shanxi Maternal and Child Health Hospital,Taiyuan,Shanxi 030013,China)

机构地区：[1]山西省妇幼保健院乳腺外科,山西太原030013 [2]山西省妇幼保健院影像中心,山西太原030013

出　　处：《转化医学杂志》2024年第11期1820-1824,共5页Translational Medicine Journal

基　　金：山西省基础研究计划项目(202103021224436)。

摘　　要：目的探讨miR-30a-5p对乳腺癌细胞中人类表皮生长因子受体2(HER2)基因及肿瘤转移抑制基因1(TMSG-1)蛋白表达的影响。方法选择人乳腺癌MCF-7细胞,将其分为Mm组(MCF-7细胞转染miR-30a-5p mimic)、Nc组(MCF-7细胞转染miR-30a-5p阴性对照)、Mc组(MCF-7细胞),运用逆转录-聚合酶链反应(RT-PCR)法、Westernblot法对HER2、TMSG-1水平变化、蛋白表达进行检测,小室法检测细胞迁移能力,四唑盐比色试验法检测MCF-7细胞增殖情况,流式细胞仪检测MCF-7细胞凋亡情况。结果Nc组与Mc组HER2、TMSG-1水平比较差异无统计学意义(P均>0.05);相较于Nc组、Mc组,Mm组HER2水平下降,TMSG-1水平升高(P均<0.05)。Nc组McF-7细胞凋亡率(7.43±0.92)与Mc组(7.40±1.03)比较差异无统计学意义(P>0.05);Mm组McF-7细胞凋亡率(23.63±3.15)明显高于Nc组和Mc组(P均<0.05)。Nc组与Mc组MCF-7细胞迁移能力比较差异无统计学意义(P>0.05),Mm组MCF-7细胞迁移能力较Nc组、Mc组明显降低(P均<0.05)。Nc组与Mc组MCF-7细胞增殖能力比较差异无统计学意义(P>0.05),相较于Nc组、Mc组,Mm组MCF-7细胞活力减弱(P均<0.05)。结论mi R-30a-5p过表达可使MCF-7细胞大量凋亡,其作用机制可能通过降低HER2、提高TMSG-1水平实现。Objective To investigate the effect of miR-30a-5p on the expression of Human Epidermal Growth Factor Receptor 2(HER2)gene and Tumor Metastasis Suppressor Gene 1(TMSG-1)protein in breast cancer cells.Methods Human breast cancer MCF-7 cells were divided into the Mm group(MCF-7 cells transfected with miR-30a-5p mimic),the Nc group(MCF-7 cells transfected with miR-30a-5p negative control),and the Mc group(MCF-7 cells).Reverse Transcription-Polymerase Chain Reaction(RT-PCR)and Western blot were used to detect the changes in HER2 and TMSG-1 levels and protein expression.Cell migration ability was detected by the transwell assay,MCF-7 cell proliferation was detected by the tetrazolium salt colorimetric assay,and MCF-7 cell apoptosis was detected by flow cytometry.Results There was no significant difference in HER2 and TMSG-1 levels between the Nc group and the Mc group(P>0.05).Compared with the Nc group and the Mc group,HER2 levels in the Mm group were decreased,and TMSG-1 levels were increased(P<0.05).There was no significant difference in HER2 and TMSG-1 protein expression between the Nc group and the Mc group(all P>0.05).Compared with the Nc group and the Mc group,HER2 expression in the Mm group was decreased,and TMSG-1 expression was increased(all P<0.05).There was no significant difference in the apoptosis rate of MCF-7 cells between the Nc group and the Mc group(all P>0.05).The apoptosis rates of MCF-7 cells in the Nc group and the Mc group were(7.43±0.92)%and(7.40±1.03)%,respectively,while the apoptosis rate in the Mm group was(23.63±3.15)%,which was significantly higher than that in the Nc group and the Mc group(all P<0.05).There was no significant difference in the migration ability of MCF-7 cells between the Nc group and the Mc group(P>0.05),but the migration ability of MCF-7 cells in the Mm group was significantly lower than that in the Nc group and the Mc group(P<0.05).There was no significant difference in the proliferation ability of MCF-7 cells between the Nc group and the Mc group(P>0.05).Compared wit

关 键 词：乳腺癌 miR-30a-5p HER2 TMSG-1 

分 类 号：R737.9[医药卫生—肿瘤]