Twist1基因甲基化在膀胱癌辅助诊断的临床价值  

The clinical value of Twist1 gene methylation in the auxiliary diagnosis of bladder cancer

作　　者：孙强 刘志鹏 徐龙 Sun Qiang;Liu Zhipeng;Xu Long(Department of Urology,Tangshan Nanhu Hospital,Hebei Tangshan,063000)

机构地区：[1]唐山南湖医院泌尿外科,河北唐山063000

出　　处：《转化医学杂志》2024年第11期1843-1846,共4页Translational Medicine Journal

基　　金：河北省医学科学研究课题(20211167)。

摘　　要：目的探究Twist1基因甲基化在膀胱癌辅助诊断的临床价值。方法TCGA数据库比较肿瘤和对应癌旁组织之间Twist1表达水平,并评估扭曲家族碱性螺旋-环-螺旋转录因子1(Twist1)表达水平的预后价值。通过甲基化特异性聚合酶链式反应(MSP)验证膀胱癌患者与健康人中Twist1基因甲基化水平,比较临床诊断金标准与MSP检测结果的灵敏度和特异性。结果膀胱癌中Twist1的表达水平明显高于对应的癌旁组织。年龄对Twist1高表达组与低表达组的膀胱癌的总生存期具有明显相关性(P<0.001)。在膀胱癌尿液中检测到Twist1基因甲基化表达水平高于健康人(P<0.01)。膀胱镜检查与MSP检测结果具有良好的灵敏度和特异性(曲线下面积AUC=0.877)。结论Twist1基因在膀胱癌中高甲基化表达,在膀胱癌尿液中检测到Twist1基因甲基化表达水平高于健康人,非侵入性的Twist1基因甲基化检测为膀胱癌初诊提供了辅助诊断方法。Objective To explore the clinical value of Twist1 gene methylation in the auxiliary diagnosis of bladder cancer.Methods The TCGA database was used to compare the expression level of Twist1 between tumors and corresponding adjacent tissues,and to evaluate the prognostic value of Twist1 expression level.Methylation-specific PCR was used to verify the methylation level of the Twist1 gene in bladder cancer patients and healthy people,and to compare the sensitivity and specificity of the clinical diagnostic gold standard and MSP detection results.Results The expression level of Twist1 in bladder cancer was significantly higher than that in the corresponding adjacent tissues.Age had a significant correlation with the overall survival period of bladder cancer in the high-expression group and low-expression group of Twist1(P<0.001).The methylation expression level of the Twist1 gene detected in the urine of bladder cancer was higher than that of healthy people(P<0.01).Cystoscopy and MSP detection results had good sensitivity and specificity(AUC=0.877).Conclusion The Twist1 gene is highly methylated in bladder cancer.The methylation expression level of the Twist1 gene detected in the urine of bladder cancer is higher than that of healthy people.The non-invasive methylation detection of the Twist1 gene provides an auxiliary diagnostic method for the initial diagnosis of bladder cancer.

关 键 词：膀胱癌 Twist1基因 甲基化 尿液 MSP 

分 类 号：R737.14[医药卫生—肿瘤]