富硒魔芋多糖增强免疫功能研究  

Enhancing Immune Function of Selenium-Rich Konjac Polysaccharide

作　　者：李亚杰 问小龙 龙澜 向极钎 王林元[2] 邹迎春 LI Yajie;WEN Xiaolong;LONG Lan;XIANG Jiqian;WANG Linyuan;ZOU Yingchun(Enshi Tujia&Miao Autonomous Prefecture Academy of Agricultural Sciences,Enshi 445000,China;Beijing University of Chinese Medicine,Beijing 102400,China)

机构地区：[1]恩施土家族苗族自治州农业科学院,湖北恩施445000 [2]北京中医药大学,北京102400

出　　处：《食品科技》2025年第1期179-186,共8页Food Science and Technology

基　　金：湖北省重点研发计划项目(2021BBA262)。

摘　　要：目的:研究富硒魔芋多糖的免疫增强作用。方法:采用高、中、低3种剂量富硒魔芋多糖(300、200、100 mg/kg BW)灌胃小鼠,进行小鼠迟发型变态反应测定试验、伴刀豆球蛋白A(Concanavalin A,ConA)诱导的小鼠脾淋巴细胞转化试验、自然杀伤(Natural killer,NK)细胞活性测定试验、小鼠碳廓清试验、小鼠腹腔巨噬细胞吞噬鸡红细胞试验、抗体生成细胞检测试验、血清溶血素的测定试验。结果:对比无处理的阴性对照组,结果显示:高剂量组在绵羊红细胞引发的迟发型变态反应中,左后爪垫厚度显著增加(P<0.01);高剂量组于Con A刺激下的脾淋巴细胞增殖OD差值显示出高度显著差异(P<0.01);高、中剂量组的溶血空斑数量显著增多(P<0.01);高剂量组观察到血清溶血素水平、吞噬指数a及NK细胞活性均有显著提高(P<0.05)。结论:依据《保健食品功能检验与评价方法(2023年版)》可以判定富硒魔芋多糖有助于增强免疫功能,动物试验结果为阳性,为富硒魔芋多糖保健食品的开发提供了科学依据。Objective:To study the immune enhancement effect of selenium-rich konjac polysaccharide.Method:Mice were administrated with high,medium and low doses of Se-rich konjac polysaccharide(300,200,100 mg/kg BW).The tests of delayed allergic reaction,spleen lymphocyte transformation induced by concanavalin A(ConA),natural killer(NK)cell activity,carbon clearance,peritoneal macrophages phagocytosis of chicken red blood cells,antibody producing cells and serum hemolysin were performed.Results:Compared with the negative control group without treatment,the results showed that the thickness of left hind paw pad increased significantly in delayed type hypersensitivity(DTH)reaction induced by sheep red blood cell(SRBC)in high-dose group(P<0.01).OD difference of splenic lymphocyte proliferation under ConA stimulation showed highly significant in high dose group(P<0.01).The number of hemolytic plaque in high and medium dose groups was significantly increased(P<0.01).Serum hemolysin level,phagocytic index a and NK cell activity were significantly increased in high dose group(P<0.05).Conclusion:According to the“Health Food Function Test and Evaluation Method(2023 edition)”,it can be judged that selenium-rich konjac polysaccharide can enhance the immune function,animal test results are positive,which provides a scientific basis for the development of selenium-rich konjac polysaccharide health food.

关 键 词：硒 魔芋 多糖 免疫力 

分 类 号：TS201.2[轻工技术与工程—食品科学]