马铃薯GH3基因家族成员的鉴定及表达分析  

Identification and Expression Analysis of GH3 Gene Family Members in Potato(Solanum tuberosum)

作　　者：朱迪 王冰冰 贺苗苗 ZHU Di;WANG Bing-Bing;HE Miao-Miao(Academy of Agriculture and Forestry Sciences(Qinghai Academy of Agriculture and Forestry Sciences)/Key Laboratory of Qinghai-Tibetan Plateau Biotechnology of Ministry of Education/Key Laboratory of Potato Breeding of Qinghai,Engineering Research Center of Potato in Northwest Region Ministry of Education,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学农林科学院(青海省农林科学院)/青藏高原生物技术教育部重点实验室/青海省马铃薯育种重点实验室/西北马铃薯教育部工程研究中心,西宁810016

出　　处：《农业生物技术学报》2025年第3期498-512,共15页Journal of Agricultural Biotechnology

基　　金：青海省科技厅基础研究计划项目(2022-ZJ-712);国家马铃薯产业技术体系(CARS-09)。

摘　　要：GH3 (Gretchen Hagen 3)基因的表达对植物的生长发育和生物胁迫响应起到重要的作用。本研究以马铃薯(Solanum tuberosum)品种'青薯9号'为材料,对其GH3家族成员进行鉴定及生物信息学分析,同时分析其在不同马铃薯组织中以及水杨酸(salicylic acid, SA)、茉莉酸甲酯(methyl jasmonate, MeJA)、赤霉素(gibberellin A3, GA3)和晚疫病菌(Phytophthora infestans)胁迫处理下的表达模式。启动子顺式作用元件分析表明,StGH3家族成员含有大量与激素有关的作用元件,推测其具有潜在的响应生物胁迫和非生物胁迫的功能。组织特异性分析表明,StGH3.4和StGH3.5基因在根中高表达,StGH3.8基因在茎中高表达,StGH3.10和StGH3.16基因在叶片中高表达。在SA、MeJA、GA3和晚疫病菌胁迫处理下,StGH3家族成员均受到调控,且表现出不同的表达模式。StGH3.1、StGH3.5和StGH3.8基因在晚疫病菌处理下的响应更为强烈。本研究为StGH3功能的深入研究提供了参考依据。The expression of Gretchen Hagen 3(GH3)genes plays an important role in plant growth and development and response to biotic stresses.In this study,the potato(Solanum tuberosum)cultivar'Qingshu 9'as the materical for identification and bioinformatics analysis of GH3 family members,and their expression patterns were analysed in potato different tissues and in response to salicylic acid(SA),Methyl jasmonate(MeJA)and gibberellin A3(GA3)and Phytophthora infestans stresses.Promoter cis-acting element analyses showed that StGH3 family members contained a large number of hormone-related acting elements,speculated that it had potential function in response to biotic and abiotic stresses.Tissue specific analysis showed that StGH3.4 and StGH3.5 genes were highly expressed in roots,StGH3.8 was highly expressed in stems,and StGH3.10 and StGH3.16 were highly expressed in leaves.StGH3 family members were regulated under SA,MeJA,GA3 and P.infestans stress treatments and showed different expression patterns.The StGH3.1,StGH3.5,and StGH3.8 genes responded more strongly to the P.infestans treatment.This study provides a scientific basis for subsequent studies of StGH3 genes function.

关 键 词：马铃薯 GH3基因家族 激素 晚疫病菌 表达分析 

分 类 号：S336[农业科学—作物遗传育种]