银杏R2R3MYB基因家族鉴定及在花青素合成中的功能  

Identification of R2R3MYB Gene Family and Its Function in Anthocyanin Synthesis in Ginkgo biloba

作　　者：张斌[1] ZHANG Bin(Hunan Provincial Engineering Research Center for Ginkgo biloba,Hunan University of Science and Engineering,Yongzhou 425199,China)

机构地区：[1]湖南科技学院湖南省银杏工程技术研究中心,永州425199

出　　处：《农业生物技术学报》2025年第3期561-569,共9页Journal of Agricultural Biotechnology

基　　金：湖南省自然科学基金项目(2024JJ7190)。

摘　　要：MYB转录因子是植物中家族成员最多的基因家族之一,其中R2R3MYB在花青素合成过程发挥重要功能。为了从基因组水平鉴定银杏(Ginkgo biloba) R2R3MYB家族成员,同时分析其在花青素合成中的功能,本研究利用同源比对获得银杏R2R3MYB家族成员的序列信息,通过生物信息分析软件进行保守结构域分析、进化树构建和保守基序分析;利用qPCR检测基因相对表达量;通过本氏烟草(Nicotiana benthamiana)叶片转化进行基因功能研究。在银杏基因组中鉴定到78个R2R3MYB家族成员,在进化树上分为7个分支;筛选出1个与拟南芥(Arabidopsis thaliana) AtMYB75同源的基因Gb_34086,其表达受光照诱导上调;Gb_34086蛋白定位在细胞核且具有转录激活活性;过表达Gb_34086基因的本氏烟草叶片可增强花青素的积累。本研究初步证明Gb_34086在花青素合成中发挥正调节作用,为进一步研究Gb_34086作用的分子机理和提高银杏花青素产量提供了依据。MYB transcription factor is one of the gene families with the largest number of members in plants,among which R2R3MYB plays an important role in anthocyanin synthesis.The study aimed to identify R2R3MYB gene family members at genomic level and analyze their functions in anthocyanin synthesis in Ginkgo biloba.The sequence information was obtained by homology alignment,and conserved domain analysis,evolutionary tree construction and conserved motif analysis were carried out by bioinformatics analysis software.The gene expression level was detected by qPCR.The gene function was studied through tobacco(Nicotiana benthamiana)leaf transformation.78 R2R3MYB family members were identified in G.biloba genome,which were divided into 7 branches in the phylogenetic tree.One gene,Gb_34086,homologous to AtMYB75 of Arabidopsis thaliana,was selected and its expression was up-regulated by light induction.Gb_34086 protein was located in the nucleus and had transcriptional activation activity.The overexpression of Gb_34086 gene in tobacco leaves could enhance anthocyanin accumulation under light.This study preliminarily proved that Gb_34086 played a positive regulatory role in anthocyanin synthesis,which provides a basis for further study of the molecular mechanism of Gb_34086 and improving the the anthocyanin production of Ginkgo biloba.

关 键 词：银杏 R2R3MYB 转录因子 花青素 

分 类 号：S685.99[农业科学—观赏园艺]