不同光质对3种松树种子萌发及幼苗生长与HY5基因表达的影响  

Effects of Different Lights on Seed Germination,Seedling Growth and HY5 Gene Expression of 3 Pine Species

作　　者：朱梅彩 刘子腾 甘沛华 李培玲 纵丹[1] 何承忠[1] Zhu Meicai;Liu Ziteng;Gan Peihua;Li Peiling;Zong Dan;He Chengzhong(Key Laboratory of National Forestry Administration on Biodiversity Conservation in Southwest China/Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China,Southwest Forestry University,Kunming Yunnan 650233,China)

机构地区：[1]西南林业大学云南省高校林木遗传改良与繁育重点实验室,西南林业大学西南地区生物多样性保育国家林业和草原重点实验室,云南昆明650233

出　　处：《西南林业大学学报(自然科学)》2025年第1期10-17,共8页Journal of Southwest Forestry University:Natural Sciences

基　　金：云南省应用基础研究计划重点项目(2017FA011)资助;中央引导地方科技发展专项资金项目(YDZX201953000002845)资助;云南省专家工作站建设项目(202005AF150020)资助。

摘　　要：选取云南不同海拔的思茅松、云南松和高山松主要荒山造林树种为研究对象,以红光、蓝光和白光为光源,测定种子萌发、幼苗生长和生物量积累的相关参数,并分析了幼苗下胚轴伸长与HY5表达的关系。结果表明:与白光相比,红光对3种松树种子萌发和幼苗生长均有促进作用,其中思茅松分别提高了23.70%和14.02%,云南松提高了11%和11.61%,高山松提高了9.37%和11.35%,但降低了幼苗针叶叶绿素含量,且对生物量积累无显著影响;蓝光提高了3种松树幼苗的叶绿素含量与生物量蓄积,其中生物量蓄积在思茅松、云南松和高山松中分别提高了3.27%、11.72%和2.28%,但在种子萌发及幼苗生长方面对云南松和高山松有抑制作用,对思茅松有促进作用;红光和蓝光光照下,3种松树幼苗下胚轴伸长与HY5相对表达量呈负相关。思茅松种子萌发及幼苗生长在不同光质下表现出红光>蓝光>白光,而云南松和高山松则表现出红光>白光>蓝光。The main afforestation trees for barren mountains at different altitudes in Yunnan,namely Pinus kesiya var.Langbianensis,Pinus yunnanensis and Pinus densata,were selected as the objects of this study.The relevant parameters of seed germination,seedling growth and biomass accumulation were determined by using RL,BL and WL(CK)as the light sources.Furthermore,the relationship between hypocotyl elongation and HY5 expression was analyzed.The results showed that RL can promote the seed germination and seedling growth of 3 pine trees,which increased by 23.70%and 14.02%for P.kesiya var.langbianensis,11%and 11.61%for P.yun-nanensis,9.37%and 11.35%for P.densata,respectively.However,the chlorophyll content of needles in three pine seedlings(P.kesiya,P.yunnanensis and P.densata decreased by 15.30%and 8.78%,respectively)was re-duced under the RL,and RL had no significant effects on biomass accumulation.The chlorophyll content and bio-mass accumulation of three pine seedlings were increased under BL,which increased by 3.27%,11.72%and 2.28%in P.kesiya var.langbianensis,P.yunnanensis and P.densata,respectively.On the other hand,it inhibited the seed germination and seedling growth of P.yunnanensis and P.densata,but promoted the growth of P.kesiya var.langbianensis.The hypocotyl elongation was negatively correlated with HY5 expression in three pine seed-lings under RL and BL light.The seed germination and seedling growth of P.kesiya var.langbianensis showed RL>BL>WL under different light quality,while that of P.yunnanensis and P.densata showed RL>WL>BL.

关 键 词：思茅松 云南松 高山松 红蓝光 种子萌发 幼苗生长 HY5 

分 类 号：S603.2[农业科学—园艺学]