创伤后应激障碍模型小鼠海马和前额叶皮质髓鞘碱性蛋白表达变化  

Changes in myelin basic protein expression in the hippocampus and prefrontal cortex of model mice with post-traumatic stress disorder

作　　者：李吉鑫 胡敏[1] 胡媛媛 孟瑶 张桂青[1] Li Jixin;Hu Min;Hu Yuanyuan;Meng Yao;Zhang Guiqing(Department of Clinical Psychology,the First Affiliated Hospital of Shihezi University,Shihezi 832008,China;Rehabilitation Medicine Center,the First Affiliated Hospital of Shihezi University,Shihezi 832008,China)

机构地区：[1]石河子大学第一附属医院临床心理科,石河子832008 [2]石河子大学第一附属医院康复医学中心,石河子832008

出　　处：《中华行为医学与脑科学杂志》2025年第2期97-103,共7页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金(32260208);石河子大学第一附属医院博士基金项目(BS202210);新疆生产建设兵团2022年度"天池英才"引进计划青年博士项目。

摘　　要：目的探究创伤后应激障碍(PTSD)模型小鼠行为学及海马、前额叶皮质髓鞘碱性蛋白(MBP)的变化。方法32只清洁级雄性C57BL/6J小鼠按照体质量相匹配方法分为对照组和PTSD 3 d组、PTSD 7 d组和PTSD 14 d组,每组8只。PTSD 3 d组、PTSD 7 d组和PTSD 14 d组小鼠分别在造模后3 d、7 d、14 d处死。PTSD组小鼠采用条件性足部电击和单次延长刺激来制备PTSD动物模型。通过旷场实验(OFT)检测小鼠的焦虑和抑郁样反应。通过小鼠穿梭被动回避实验进行电击刺激训练和记忆保持测试。通过髓鞘染色观察小鼠海马和前额叶皮质的髓鞘形态学变化。利用Western blot和免疫组织化学染色检测MBP的表达。采用GraphPad Prism 8.0.2和SPSS 25.0软件分析实验数据和作图,行为学和分子指标数据的多组间比较采用单因素方差分析,MBP蛋白相对表达量与行为学指标的相关性采用Pearson相关分析。结果(1)行为学结果:各组小鼠旷场实验活动总路程、平均速度、不动时间和进入中央区域次数比较均差异有统计学意义(F=16.72,16.74,7.37,5.93,均P<0.05)。PTSD 3 d、7 d、14 d组活动总路程、平均速度均低于对照组(均P<0.05);在小鼠穿梭被动回避实验中,各组小鼠活动总路程、平均速度、进入暗箱次数和活动时间比较均差异有统计学意义(F=52.78,55.47,9.07,24.88,均P<0.05)。PTSD 3 d组和PTSD 7 d组小鼠各指标均高于对照组和PTSD 14 d组(均P<0.05)。(2)髓鞘染色显示,各组小鼠海马CA1区和前额叶皮质髓鞘染色面积比较均差异有统计学意义(F=6.67,5.77,均P<0.05)。PTSD 3 d组小鼠海马CA1区和前额叶皮质髓鞘染色面积[(123.30±11.62)μm^(2),(119.50±9.26)μm^(2)]均低于对照组[(153.00±2.61)μm^(2),(154.80±12.40)μm^(2),均P<0.05]。(3)Western blot结果显示,各组小鼠海马和前额叶皮质组织MBP的表达均差异有统计学意义(F=10.15,5.28,均P<0.05)。PTSD 3 d组(0.89±0.07)、PTSD 7 d组(0.90±0.14)和PTSD 14 d组(ObjectiveTo investigate changes in behavior and hippocampal and prefrontal cortical myelin basic protein(MBP)in model mice with post-traumatic stress disorder(PTSD).MethodsThirty-two clean-grade male C57BL/6J mice were randomly divided into control group,PTSD 3 d group,PTSD 7 d group and PTSD 14 d group based on matching body mass,with 8 mice in each group.The mice in PTSD 3 d,7 d and 14 d groups were decapitated at 3 d,7 d,and 14 d after modeling.The PTSD mouse model was established through conditioned foot shock and single prolonged stress.Anxious-and depressive-like behaviors of mice were assessed by the open field test(OFT).The mouse step-through passive avoidance experiment was used for electric shock training and learning and memory retention.Myelin morphology alterations in the hippocampus and prefrontal cortex of the mouse were examined via myelin staining.The expression levels of MBP were quantified using Western blot and immunohistochemical staining techniques.All experimental data were analyzed and visualized using GraphPad Prism 8.0.2 and SPSS 25.0,the multi groups comparison of behavioral and molecular indicator data was conducted by one-way ANOVA,and the correlation between MBP relative protein expression level and behavioral indicators was analyzed by Pearson correlation analysis.Results(1)Behavioral results:in OFT,there were significant differences in total distance,average speed,time of stationary and times of entering the central area among all groups(F=16.72,16.74,7.37,5.93,all P<0.05).The total distance and the average speed time of PTSD 3 d,7 d,14 d group were all lower than those in control group(all P<0.05).In the mouse step-through passive avoidance experiment,significant differences were observed across all groups in terms of total distance traveled,average speed,dark box entries,and activity time(F=52.78,55.47,9.07,24.88,all P<0.05).All the indexes of PTSD 3 d group and PTSD 7 d group were all higher than those in control group and PTSD 14 d group(all P<0.05).(2)Myelin staining revealed

关 键 词：创伤后应激障碍 髓鞘碱性蛋白 海马 前额叶皮质 小鼠 

分 类 号：R74[医药卫生—神经病学与精神病学]