葡萄糖酸钙锌口服溶液对去卵巢大鼠骨质疏松症的改善作用  

Effect of calcium and zinc gluconate oral solution on osteoporosis in ovariectomized rats

作　　者：段娟慧 王勇 DUAN Juanhui;WANG Yong(China Resources Sanjiu Medical&Pharmaceutical Co.,Ltd,Shenzhen 518110,Guangdong Province,China)

机构地区：[1]华润三九医药股份有限公司,广东深圳518110

出　　处：《世界临床药物》2025年第1期38-43,共6页World Clinical Drug

摘　　要：目的探讨葡萄糖酸钙锌口服溶液对去卵巢致骨质疏松大鼠的治疗作用。方法将72只SD大鼠随机分为假手术组、模型组、阳性对照组以及锌钙特低、中、高剂量(3、6、12 ml/kg)组,每组12只。除假手术组外,其余各组大鼠去除卵巢建立骨质疏松模型。阳性对照组给予雌二醇0.1 mg/kg,假手术组和模型对照组灌胃等体积纯化水,治疗99 d。每3 d观察大鼠体质量;治疗后,采用micro-CT分析股骨骨密度(bone mineral density,BMD)、骨体积分数(bone volume relative to tissue volume,BV/TV)、骨表面积组织体积比(bone surface relative to tissue volume,BS/TV);酶联免疫吸附试验检测钙、锌和骨代谢指标。结果与模型组比较,锌钙特组显著抑制大鼠体质量增长,锌水平升高,股骨骨丢失改善,骨小梁数量和连接、BMD增加,BV/TV、BS/TV升高,骨钙素、Ⅰ型前胶原羧基末端前肽水平下降。结论锌钙特可改善去卵巢大鼠骨小梁微结构破坏,增加骨密度,减缓去卵巢大鼠骨质疏松进展,对去卵巢骨质疏松症大鼠具有良好疗效。Objective To investigate the therapeutic effect of calcium and zinc gluconate oral solution on osteoporosis in ovariectomized rats.Methods 72 SD rats were randomly divided into sham operation group,model group,positive control group and low,medium and high doses of Xingaite groups(3,6,12 ml/kg),with 12 rats in each group.Except for the sham operation group,the ovaries were removed to establish the osteoporosis model.The positive control group was given estradiol 0.1 mg/kg,and the sham operation group and the model control group were given the same volume of purified water by gavage for 99 days.The body mass of the rats was observed every 3 days.After treatment,bone mineral density(BMD),bone volume relative to tissue volume(BV/TV)and bone surface relative to tissue volume(BS/TV)were analyzed by micro-CT.Calcium,zinc and bone metabolism were detected by enzyme linked immunosorbent assay.Results Compared with the model group,the zinc-calcium group significantly inhibited the growth of body mass,increased the level of zinc,improved the bone loss of femur,increased trabecular number and connection and BMD,increased BV/TV and BS/TV,and decreased the levels of osteocalcin and procollagen typeⅠcarboxy-terminal peptide.Conclusion Xingaite can improve the microstructure of bone trabecular in ovariectomized rats,increase bone density,slow down the osteoporosis process in ovariectomized rats,and has a good effect on ovariectomized rats with osteoporosis.

关 键 词：葡萄糖酸钙锌口服溶液 骨质疏松症 去卵巢模型 

分 类 号：R-33[医药卫生]