甜瓜β-淀粉酶基因家族的鉴定及对非生物胁迫的响应  

Identification ofβ-amylase Gene Family in Melon and Their Response to Abiotic Stresses

作　　者：韩江涛 张帅博 秦雅蕊 韩硕洋 张雅康 王吉庆[1] 杜清洁 肖怀娟 李猛 HAN Jiang-tao;ZHANG Shuai-bo;QIN Ya-rui;HAN Shuo-yang;ZHANG Ya-kang;WANG Ji-qing;DU Qing-jie;XIAO Huai-juan;LI Meng(College of Horticulture of Henan Agricultural University,Zhengzhou 450046)

机构地区：[1]河南农业大学园艺学院,郑州450046

出　　处：《生物技术通报》2025年第3期171-180,共10页Biotechnology Bulletin

基　　金：国家自然科学基金项目(32202578);中国博士后科学基金项目(2022M711063);河南省大宗蔬菜产业技术体系岗位专家项目(HARS-22-07-G4)。

摘　　要：【目的】β-淀粉酶(β-amylase,BAM)能水解淀粉,在植物生长和响应非生物胁迫中起重要作用。从全基因组水平对甜瓜BAM基因家族成员进行鉴定,为甜瓜BAM基因功能研究及抗性育种提供基因资源。【方法】分析BAM家族蛋白理化性质、系统进化、基因结构、保守基序、启动子顺式作用元件以及通过RT-qPCR对其在干旱、ABA、低温、盐胁迫下的表达模式进行分析。【结果】在甜瓜全基因组中鉴定出9个CmBAM基因家族成员,分别位于第1、4、5、6、7和11号染色体上,蛋白平均氨基酸个数为543,平均分子量为58.7 kD,等电点为5.47–8.89。系统进化分析表明,甜瓜BAM基因家族成员与黄瓜的亲缘关系最近,同源性在94.5%以上,且存在一一对应的关系。甜瓜CmBAM成员启动子中主要顺式作用元件为激素响应元件、光响应元件、非生物胁迫元件和蛋白质结合元件。CmBAMs在干旱、ABA、低温和盐处理下的表达模式分析发现,CmBAMs不同程度地响应4种非生物胁迫,其中CmBAM1、CmBAM3和CmBAM9受干旱强烈诱导,CmBAM3、CmBAM5、CmBAM9受ABA强烈诱导,CmBAM1、CmBAM5、CmBAM9受低温胁迫强烈诱导;CmBAM3的表达受盐胁迫强烈诱导。【结论】从甜瓜全基因组中鉴定出9个CmBAMs,CmBAM1、CmBAM3、CmBAM5和CmBAM9对4种非生物胁迫最为敏感,表明其可能在非生物胁迫中发挥关键作用,可作为后续研究的候选基因。【Objective】β-amylase(BAM)catalyzes the hydrolysis of starch and plays a crucial role in plant growth and stress response.Identifying the BAM gene family members from the whole melon genome may provide genetic resources for function study of BAM gene and resistance breeding in melon.【Method】This work analyzed the physicochemical properties,phylogenetic evolution,gene structure,conserved motifs,promoter cis-acting regulatory elements of the BAM family’s protein.RT-qPCR was used to analyze their expression patterns under drought,ABA,low temperature,and salt stress.【Result】Nine CmBAMs were identified from the whole-genome of melon(Cucumis melo),and they were located on chromosome 1,4,5,6,7,and 11,with an average length of 543 amino acids.The average molecular weight was approximately 58.7 kD,and the isoelectric point ranged from 5.47 to 8.89.Phylogenetic analysis demonstrated that BAMs in melon were highly homologous with cucumbers(Cucumis sativus)with over 94.5%homology and one-to-one correspondence.CmBAMs promoter in melon mainly harbored hormone-responsive elements,photo-responsive elements,abiotic stress elements,and protein binding elements.Expression pattern analysis of CmBAMs under drought,ABA,low temperature,and salt stresses revealed that CmBAMs responded to these four abiotic stresses at varied level.Notably,CmBAM1,CmBAM3,and CmBAM9 were dramatically induced by drought,while CmBAM3,CmBAM5,and CmBAM9 showed strong induction by ABA.CmBAM1,CmBAM5,and CmBAM9 were obviously induced by cold stress whereas CmBAM3 showed the highest induction under salt stress.【Conclusion】Nine CmBAMs are identified from the whole genome of melon,and CmBAM1,CmBAM3,CmBAM5,and CmBAM9 were the most sensitive to the four abiotic stresses,suggesting that they might play a key role in abiotic stress and thus can be selected as candidate genes for further research.

关 键 词：甜瓜 Β-淀粉酶 基因家族 生物信息学 非生物胁迫 

分 类 号：S65[农业科学—果树学]