基于超高效液相色谱-串联质谱法的一测多评法同时测定穿心莲中9种成分含量  

Simultaneous determination of 9 kinds of components in Andrographis paniculata by one-point multi-component method based on ultra performance liquid chromatography-tandem mass spectrometry

作　　者：陈少华 黄曦 余开科 朱飞如 郑燕 李峰 刘莉丽 叶有芳 黄梓霞 梁国华 CHEN Shao-Hua;HUANG Xi;YU Kai-Ke;ZHU Fei-Ru;ZHENG Yan;LI Feng;LIU Li-Li;YE You-Fang;HUANG Zi-Xia;LIANG Guo-Hua(Beihai Food and Drug Safety Certification and Evaluation Center,Beihai 536000,China;Beihai Public Inspection and Testing Center,Beihai 536000,China)

机构地区：[1]北海市食品药品安全认证评审中心,北海536000 [2]北海市公共检验检测中心,北海536000

出　　处：《食品安全质量检测学报》2025年第6期122-131,共10页Journal of Food Safety and Quality

基　　金：广西壮族自治区市场局自筹课题项目(GSJKJZC2024-9)。

摘　　要：目的 建立一测多评(quantitative analysis of multi-components by single marker, QAMS)法同时测定穿心莲中9种成分含量的分析方法。方法 采用超高效液相色谱-串联质谱法,以穿心莲内酯为内参物,建立该成分与连翘苷、齐墩果酸、咖啡酸、穿心莲内酯、脱水穿心莲内酯、穿心莲新酯、去氧穿心莲内酯、脱水穿心莲内酯琥珀酸半酯、亚硫酸氢钠穿心莲内脂的相对校正因子,利用相对校正因子来计算各成分的含量,同时将QAMS法计算结果和外标法实测值进行比较。结果 相对校正因子重现性良好,使用QAMS法与外标法所测得的结果无明显的差异。结论 QAMS法适用于穿心莲中9种成分的同时测定,以保证质量的可靠性。Objective To establish a quantitative analysis of multi-components by single marker(QAMS)method for the simultaneous determination of 9 kinds of components in Andrographis paniculata.Method The relative calibration factors of andrographolide with forsythitin,oleanolic acid,caffeic acid,andrographolide,dehydrated andrographolide,andrographolide,deoxyandrographolide,dehydrated andrographolide half succinate and sodium bisulfite were established by ultra performance liquid chromatographolide-tandem mass spectrometry. The relative correction factor was used to calculate the content of each component, and the calculated results of QAMS method were compared with the measured values of external standard method. Results The relative correction factors showed good reproducibility, and there was no significant difference between the results obtained by the QAMS method and the external standard method. Conclusion The QAMS method is suitable for the simultaneous determination of 9 kinds of components in Andrographis paniculata, ensuring the reliability of quality.

关 键 词：穿心莲 超高效液相色谱-串联质谱法 一测多评法 

分 类 号：R284.1[医药卫生—中药学] O657.63[医药卫生—中医学]