EBER原位杂交检测技术专家共识  

Expert consensus on EBER in-situ hybridization detection technology

作　　者：中国医师协会医学技师委员会病理技术专家组 中国医学装备协会病理装备分会标准化部 中国抗癌协会肿瘤病理专业委员会病理技术学组 中国研究型医院学会病理学专业委员会病理技术学组 周立新 张炜明[3] 薛晓伟[4] 徐黎明[5] 丁伟[5] Pathological Technical Expert Group,Medical Technician Committee of Chinese Medical Doctor Association;Standardization Department,China Association of Pathology Equipment;Pathological Technology Group,Pathology Committee of Chinese Research Hospital Association;Pathological Technology Group,Pathological Committee of China Anti-cancer Association

机构地区：[1]不详 [2]北京大学肿瘤医院 [3]江苏省人民医院 [4]中国医学科学院北京协和医院 [5]浙江大学医学院附属第一医院

出　　处：《临床与实验病理学杂志》2025年第2期157-161,共5页Chinese Journal of Clinical and Experimental Pathology

摘　　要：EBER原位杂交检测技术是一种用于检测RNA分子在组织细胞中表达定位的方法,规范检测是其结果准确性的根本保证。针对EBER原位杂交检测技术在临床应用中的关键问题,中国医师协会医学技师委员会病理技术专家组、中国医学装备协会病理装备分会标准化部、中国抗癌协会肿瘤病理专业委员会病理技术学组及中国研究型医院学会病理学专业委员会病理技术学组组织相关领域专家进行深入探讨,最终形成此共识。该共识涵盖了EBER原位杂交检测技术的原理、样本处理规范、标准操作流程以及质量控制措施等方面内容,旨在为临床实验室开展EBER原位杂交检测提供指导,以提高检测结果的准确性和可靠性。EBER in-situ hybridization detection technology is a method used to detect the expression and localization of RNA molecules in tissuess and cells.Standardized detection is fundamental to ensuring the accuracy of the results.To address key issues in the clinical application of EBER in-situ hybridization detection technology,Pathological Technical Expert Group of Medical Technician Committee of Chinese Medical Doctor Association,Standardization Department of China Association of Pathology Equipment,Pathological Technology Group of Pathological Committee of China Anti-cancer Association,and Pathological Technology Group of Pathology Committee of Chinese Research Hospital Association organized experts from related fields to conduct in-depth discussions and reached this consensus.This consensus covers aspects such as the principle of EBER in-situ hybridization detection technology,sample processing standards,standards operating procedures,and quality control protocols.It aims to provide guidance for clinical laboratories to carry out EBER in-situ hybridization detection,as well as improving the accuracy and reliability of detection results.

关 键 词：原位杂交 EBER 质量控制 

分 类 号：R-331[医药卫生]