发酵液中苄胺的纯化工艺研究  

STUDY ON PURIFICATION TECHNOLOGY OF BENZYLAMINE FROM FERMENTATION BROTH

作　　者：邱警 尹靖欣 杨佳妮 赵东旭[2] 张卉 高海军[2] Qiu Jing;Yin Jingxin;Yang Jiani;Zhao Dongxu;Zhang Hui;Gao Haijun(School of Environmental and Safety Engineering,Shenyang University of Chemical Technology,Shenyang 110142,Liaoning,China;School of Life Science,Beijing Institute of Technology,Beijing 100081,China)

机构地区：[1]沈阳化工大学环境与安全工程学院,辽宁沈阳110142 [2]北京理工大学生命学院,北京100081

出　　处：《精细石油化工》2025年第2期52-56,共5页Speciality Petrochemicals

摘　　要：采用液液萃取法和减压蒸馏法对微生物发酵液中苄胺进行分离提纯。从5种有机溶剂中筛选确定二氯甲烷为萃取剂;调节pH值为11,离心除去料液中悬浮物后,对料液进行3次萃取,萃取率分别是81.3%、79.1%、56.4%,总萃取率为98.3%;减压蒸馏条件是温度30℃、真空度0.07 MPa,苄胺回收率为94%。所建立的萃取-减压蒸馏工艺可以从发酵液中高效提取苄胺,操作简单,易于放大,回收率高。This paper uses liquid-liquid extraction and vacuum distillation to separate and purify benzylamine from microbial fermentation liquid.Dichloromethane was selected as the extractant from five organic solvents after screening experiments.After adjusting the pH to 11 and removing suspended solids by centrifugation,the fermentation liquid was subjected to three rounds of extraction,with extraction rates of 81.3%,79.1%,and 56.4%,respectively,and a total extraction rate of 98.3%.The vacuum distillation was carried out at 30℃and 0.07 MPa vacuum,and the recovery rate of benzylamine was 94%.The established extraction-vacuum distillation process can efficiently extraction of benzylamine from fermentation liquid,with simple operation,easy expansion,and high recovery rate.

关 键 词：苄胺 液液萃取 减压蒸馏 二氯甲烷 发酵液 

分 类 号：O625.63[理学—有机化学]