CBL1/CIPK23 phosphorylates tonoplast sugar transporter TST2 to enhance sugar accumulation in sweet orange(Citrus sinensis)  

作　　者：Mengdi Li Zuolin Mao Zeqi Zhao Siyang Gao Yanrou Luo Ziyan Liu Xiawei Sheng Xiawan Zhai Ji-Hong Liu Chunlong Li 

机构地区：[1]National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China [2]Hubei Hongshan Laboratory,Wuhan 430070,China

出　　处：《Journal of Integrative Plant Biology》2025年第2期327-344,共18页植物学报(英文版)

基　　金：supported by National Key Research and Development Program of China(2023YFD2300603,2022YFF1003100);the National Natural Science Foundation of China(32322073);Foundation of Hubei Hongshan Laboratory(2021hszd016);Fundamental Research Funds for the Central Universities(2662023PY011);the Huazhong Agricultural University(start-up funding to C.L.)。

摘　　要：Fruit taste quality is greatly influenced by the content of soluble sugars,which are predominantly stored in the vacuolar lumen.However,the accumulation and regulation mechanisms of sugars in most fruits remain unclear.Recently,we established the citrus fruit vacuole proteome and discovered the major transporters localized in the vacuole membrane.Here,we demonstrated that the expression of tonoplast sugar transporter 2(CsTST2)is closely associated with sugar accumulation during sweet orange(Citrus sinensis)ripening.It was further demonstrated that CsTST2 had the function of transporting hexose and sucrose into the vacuole.Overexpression of CsTST2 resulted in an elevation of sugar content in citrus juice sac,calli,and tomato fruit,whereas the downregulation of its expression led to the reduction in sugar levels.CsTST2 was identified as interacting with CsCIPK23,which binds to the upstream calcium signal sensor protein CsCBL1.The phosphorylation of the three serine residues(Ser277,Ser337,and Ser354)in the loop region of CsTST2 by CsCIPK23 is crucial for maintaining the sugar transport activity of CsTST2.Additionally,the expression of CsCIPK23 is positively correlated with sugar content.Genetic evidence further confirmed that calcium and CsCIPK23-mediated increase in sugar accumulation depends on CsTST2 and its phosphorylation level.These findings not only unveil the functional mechanism of CsTST2 in sugar accumulation,but also explore a vital calcium signal regulation module of CsCBL1/CIPK23 for citrus sweetness quality.

关 键 词：CITRUS CsCBL1/CIPK23 PHOSPHORYLATION tonoplast sugar transporter VACUOLE 

分 类 号：S666.4[农业科学—果树学]