超声辅助分散液液微萃取—高效液相色谱法快速测定饮料中富马酸二甲酯含量  

Rapid determination of dimethyl fumarate in beverages by dispersed liquid-liquid microextraction with ultrasound-assisted and high performance liquid chromatography

作　　者：姜放军[1] 梁锋 冯亚龙 李秋怡 丁芳林[1] 张朝辉[1] JIANG Fangjun;LIANG Feng;FENG Yalong;LI Qiuyi;DING Fanglin;ZHANG Zhaohui(Hunan Biological and Electromechanical Polytechnic,Changsha,Hunan 410127,China;Hunan Provincial Product Quality Inspection Research Institute,Changsha,Hunan 410116,China)

机构地区：[1]湖南生物机电职业技术学院,湖南长沙410127 [2]湖南省产商品质量检验研究院,湖南长沙410116

出　　处：《食品与机械》2025年第1期80-85,共6页Food and Machinery

基　　金：湖南省自然科学基金科教联合项目(编号:2022JJ60070);湖南省教育厅科研项目(编号:21C1254)。

摘　　要：[目的]建立一种超声辅助分散液液微萃取—高效液相色谱法快速测定饮料中富马酸二甲酯含量的方法。[方法]在饮料试样中加入萃取剂与分散剂,采用超声萃取,离心分离,高效液色谱法进行测定。[结果]最佳微萃取条件为100μL氯仿作为萃取剂,300μL丙酮作为分散剂,超声时间4 min,此时富马酸二甲酯的线性范围为0.1~100.0μg/mL,检出限为0.02μg/mL。方法的相对标准偏差(RSD)<3.7%,加标回收率为75.0%~85.0%。[结论]该方法简单快速、重现性好、分析灵敏度高、环境友好,适于饮料中富马酸二甲酯的定性与定量分析。[Objective]To establish a rapid detecting method for the determination of dimethyl fumarate in beverages by ultrasound-assisted dispersive liquid-liquid micro-extraction coupled with high performance liquid chromatography.[Methods]The beverage samples were spiked with extractant and dispersant,extracted by ultrasonic extraction,centrifuged and determined by high performance liquid chromatography.[Results]The optimal microextraction conditions were 100μL of chloroform as extractant,300μL of acetone as dispersant,and a sonication time of 4 min.Under the optimal extraction conditions,dimethyl fumarate had a linear range of 0.1~100.0μg/mL with a detection limit of 0.02μg/L.The relative standard deviation(RSD)of the method was less than 3.7%,and the spike recovery rate was between 75.0%and 85.0%.[Conclusion]The method is simple,rapid,reproducible,sensitive and environmentally friendly,and is suitable for the qualitative and quantitative analysis of dimethyl fumarate in beverages.

关 键 词：分散液液微萃取 高效液相色谱 饮料 富马酸二甲酯 

分 类 号：TS27[轻工技术与工程—农产品加工及贮藏工程] O657.72[轻工技术与工程—食品科学与工程]