18F-FDG在不同类型髓系白血病细胞及其荷瘤裸鼠中的代谢特点研究  

Metabolic Characteristics of 18F-FDG in Different Types of Myeloid Leukemia Cells and Tumor-Bearing Nude Mice

作　　者：陈曦 严钦 覃祥 张利 冯悦 陈千 龙思利 刘文君 CHEN Xi;YAN Qin;QIN Xiang;ZHANG Li;FENG Yue;CHEN Qian;LONG Si-Li;LIU Wen-Jun(Department of Pediatrics,The Affiliated Hospital of Southwest Medical University,The Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China;Birth Defects and Childhood Hematological Oncology Laboratory,The Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China;Sichuan Clinical Research Center for Birth Defects,The Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China;Department of Nuclear Medicine,The Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China)

机构地区：[1]西南医科大学附属医院儿科(儿童血液肿瘤病区),四川泸州646000 [2]西南医科大学附属医院儿童血液肿瘤与出生缺陷实验室,四川泸州646000 [3]西南医科大学附属医院四川省出生缺陷临床医学研究中心,四川泸州646000 [4]西南医科大学附属医院核医学科,四川泸州646000

出　　处：《中国实验血液学杂志》2025年第2期325-330,共6页Journal of Experimental Hematology

基　　金：四川省科技计划资助(2022YFS0622)。

摘　　要：目的:通过体外培养髓系白血病细胞和构建荷瘤裸鼠模型,对比研究18F-氟代脱氧葡萄糖(18F-FDG)在髓系白血病中的代谢特点。方法:体外培养U937、THP-1、HL60和K562细胞,分别于18F-FDG加入后15、30、60和120min测定细胞对18F-FDG的摄取率。将4种细胞接种于裸鼠后肢皮下建立荷瘤裸鼠模型,当瘤体大小约为500 mm3时,经鼠尾静脉注射18F-FDG,在注射后60 min时行正电子发射断层成像/计算机断层扫描。采用连续病理切片苏木精-伊红染色观察荷瘤细胞形态。结果:18F-FDG与不同类型髓系白血病细胞共同孵育后,U937细胞在4个时间点的摄取率均明显高于THP-1、HL60、K562细胞(均P<0.05);THP-1细胞在4个时间点的摄取率均高于K562细胞(均P<0.05);前60 min内白血病细胞对18F-FDG的摄取速度较快,60 min之后摄取速度趋于稳定。成功构建了U937、THP-1、HL60、K562白血病细胞荷瘤裸鼠模型,U937荷瘤裸鼠对18F-FDG摄取值均明显高于THP-1、HL60、K562荷瘤裸鼠(均P<0.01);THP-1和HL60荷瘤裸鼠对18F-FDG摄取值明显高于K562荷瘤裸鼠(均P<0.01)。结论:通过皮下接种法成功构建髓系白血病荷瘤裸鼠模型,在体外培养的细胞和荷瘤裸鼠模型中,急性髓系白血病细胞对18F-FDG摄取率较高,18F-FDG可能对急性髓系白血病具有更好的临床应用价值。Objective:To investigate the metabolic characteristics of 18F-fluorodeoxyglucose(18F-FDG)in myeloid leukemia by in vitro culture of myeloid leukemia cells and construction of tumor-bearing nude mouse model.Methods:U937,THP-1,HL60 and K562 cells were cultured in vitro.The cells in logarithmic growth phase(l×105 cells/well)were added with 18F-FDG,and the uptake rate of 18F-FDG was measured at 15,30,60 and 120 min after addation,respectively.The four kinds of cells were inoculated subcutaneously into the hind limbs of nude mice to establish a tumor-bearing nude mouse model.When the tumor size was about 500 mm3,18F-FDG was injected through the tail vein of the mice,and positron emission tomography/computed tomography was performed at 60 min after injection.The morphology of tumor-bearing cells was observed by hematoxylin-eosin(HE)staining in serial pathological sections.Results:After coincubation with 18F-FDG,the 18F-FDG uptake rates of U937 cells were significantly higher than THP-1,HL60 and K562 cells at 4 time points(all P<0.05),and THP-1 cells were higher than K562 cells(all P<0.05).The uptake rate of 18F-FDG by leukemia cells was rapid in the first 60 min,then tended to be stable.Pathological analysis showed that subcutaneous inoculation of U937,THP-1,HL60 and K562 cells could successfully establish tumor-bearing nude mouse models of myeloid leukemia.The 18F-FDG uptake value in U937 tumor-bearing nude mice was significantly higher than THP-1,HL60 and K562 tumor-bearing nude mice(all P<0.01).The 18F-FDG uptake values in THP-1 and HL60 tumor-bearing nude mice were significantly higher than that in K562 tumor-bearing nude mice(both P<0.01).Conclusion:The tumor-bearing nude mouse model of myeloid leukemia can be successfully constructed by subcutaneous inoculation.The 18F-FDG uptake rate of acute myeloid leukemia(AML)cells is higher in cells cultured in vitro and tumor-bearing nude mouse model.18F-FDG may have better clinical application value for AML.

关 键 词：白血病 18F-氟代脱氧葡萄糖 荷瘤裸鼠 

分 类 号：R733.7[医药卫生—肿瘤]