lncRNAN CK1-AS1在急性髓系白血病中的表达及临床意义  

Expression and Clinical Significance of lncRNA NCK1-AS1 in Acute Myeloid Leukemia

作　　者：程晨 徐子浚 夏培慧 闻向梅[1,2] 马吉春 谷雨[2,3] 于迪 钱军 林江 CHENG Chen;XU Zi-Jun;XIA Pei-Hui;WEN Xiang-Mei;MA Ji-Chun;GU Yu;YU Di;QIAN Jun;LIN Jiang(Central Laboratory,the Affiliated People’s Hospital of Jiangsu University;Zhenjiang Clinical Research Center of Hematology;Department of Hematology,the Affiliated People’s Hospital of Jiangsu University,Zhenjiang 212002,Jiangsu Province,China)

机构地区：[1]江苏大学附属人民医院中心实验室 [2]镇江市血液病临床研究中心 [3]江苏大学附属人民医院血液科,江苏镇江212002

出　　处：《中国实验血液学杂志》2025年第2期352-358,共7页Journal of Experimental Hematology

基　　金：国家自然科学基金(82270179,81970118,81900163);镇江血液学研究中心(SS2018009);镇江社会发展基金会(SH2023022,SH2022026,SH2023022);江苏大学医教协同创新基金项目(JDY2023008)。

摘　　要：目的:初步检测和分析长链非编码RNA酪氨酸激酶非催化区衔接蛋白1-反义RNA1(NCK1-AS1)在急性髓系白血病(AML)中的表达及临床意义。方法:选取江苏大学附属人民医院AML确诊患者89例和健康对照者23例,采用实时荧光定量聚合酶链式反应(RT-qPCR)方法检测两组骨髓标本中NCK1-AS1和NCK1的表达水平,分析NCK1-AS1的表达与患者的临床特征的关系,以及NCK1-AS1与NCK1之间的相关性。结果:NCK1-AS1表达水平在全部AML、非M3型AML和正常核型AML患者中均较对照组显著上调(P<0.01,P<0.05,P<0.01)。在非M3型AML中,NCK1-AS1高表达患者的血红蛋白含量低于低表达组(P=0.036),高表达组患者总体生存时间也较低表达组短(P=0.0378)。Cox多因素分析显示,NCK1-AS1表达是非M3型AML患者的一个独立预后因素(HR=2.392,95%CI:1.089-5.255,P=0.030)。与对照组相比,NCK1在全部AML、非M3型AML和正常核型AML中表达均显著上调(P<0.01,P<0.01,P<0.001)。NCK1-AS1与NCK1的表达存在一定的相关性(r=0.37,P=0.0058)。结论:NCK1-AS1在AML中高表达预示着AML患者预后不良。Objective:To detect and analyze the expression and clinical significance of long non-coding RNA tyrosine kinase non-catalytic region adaptor protein 1-antisense RNA1(NCK1-AS1)in patients with acute myeloid leukemia(AML).Methods:89 AML patients and 23 healthy controls were included from the People’s Hospital Affiliated to Jiangsu University.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression levels of NCK1-AS1 and NCK1 in bone marrow samples.The relationship between the expression of NCK1-AS1 and the clinical characteristics of patients were analyzed,as well as the correlation between NCK1-AS1 and NCK1.Results:The expression level of NCK1-AS1 in all AML,non-M3 AML and cytogenetically normal AML(CN-AML)patients was significantly higher than that in the control group(P<0.01,P<0.05,P<0.01,respectively).In non-M3 AML,patients with high NCK1-AS1 expression had a significantly lower hemoglobin level than those with low NCK1-AS1 expression(P=0.036),furthermore,NCK1-AS1 high patients had shorter overall survival than NCK1-AS1 low patients(P=0.0378).Multivariate analysis showed that NCK1-AS1 expression was an independent adverse factor in patients with non-M3 AML(HR=2.392,95%CI:1.089-5.255,P=0.030).In addition,NCK1 expression was also significantly upregulated in all AML,non-M3 AML and CN-AML patients compared with controls(P<0.01,P<0.01,P<0.001,respectively).There was a certain correlation between NCK1-AS1 and NCK1 expression(r=0.37,P=0.0058).Conclusion:High expression of NCK1-AS1 in AML indicates poor prognosis of AML patients.

关 键 词：急性髓系白血病 长链非编码RNA NCK1-AS1 

分 类 号：R733.71[医药卫生—肿瘤]