TBL1XR1突变对弥漫大B细胞淋巴瘤细胞生物学特性的影响  

Effect of TBL1XR1 Mutation on Cell Biological Characteristics of Diffuse Large B-Cell Lymphoma

作　　者：范洪明 洪乐旻 黄春群 卢金凤 徐宏慧 陈洁[1] 黄红铭[1] 王信峰[1] 郭丹 FAN Hong-Ming;HONG Le-Min;HUANG Chun-Qun;LU Jin-Feng;XU Hong-Hui;CHEN Jie;HUANG Hong-Ming;WANG Xin-Feng;GUO Dan(Department of Hematology,Affiliated Hospital of Nantong University,Nantong 226001,Jiangsu Province,China)

机构地区：[1]南通大学附属医院血液科,江苏南通226001

出　　处：《中国实验血液学杂志》2025年第2期423-430,共8页Journal of Experimental Hematology

基　　金：南通市科技项目(JC12022095)。

摘　　要：目的:探讨TBL1XR1突变对弥漫大B细胞淋巴瘤(DLBCL)细胞生物学特性的影响。方法:构建TBL1XR1过表达载体,并进行DNA测序,确定突变结果。流式细胞术及TUNEL荧光实验检测TBL1XR1突变对DLBCL细胞株凋亡的影响;CCK-8法检测TBL1XR1突变对细胞增殖的影响;Transwell实验检测TBL1XR1突变对细胞迁移及侵袭的影响;Western blot用于检测TBL1XR1突变对上皮间质转化相关蛋白表达水平的影响。结果:成功构建TBL1XR1突变的DLBCL细胞株。体外实验结果显示,TBL1XR1突变对DLBCL细胞凋亡无影响。与对照组相比,TBL1XR1突变对细胞增殖、迁移及侵袭均起到了增强作用。TBL1XR1基因突变显著增加了N-cadherin蛋白表达,而E-cadherin蛋白表达下降。结论:TBL1XR1突变在DLBCL中发挥了促进肿瘤细胞增殖、迁移和侵袭的作用。未来的研究可以考虑将T BL1XR1突变作为DLBCL治疗的新靶点。Objective:To investigate the effect of TBL1XR1 mutation on cell biological characteristics of diffuse large B-cell lymphoma(DLBCL).Methods:The TBL1XR1 overexpression vector was constructed and DNA sequencing was performed to determine the mutation status.The effect of TBL1XR1 mutation on apoptosis of DLBCL cell line was detected by flow cytometry and TUNEL fluorescence assay;CCK-8 assay was used to detect the effect of TBL1XR1 mutation on cell proliferation;Transwell assay was used to detect the effect of TBL1XR1 mutation on cell migration and invasion;Western blot was used to detect the effect of TBL1XR1 mutation on the expression level of epithelialmesenchymal transition(EMT)related proteins.Results:The TBL1XR1 overexpression plasmid was successfully constructed.The in vitro experimental results showed that TBL1XR1 mutation had no significant effect on apoptosis of DLBCL cells.Compared with the control group,TBL1XR1 mutation enhanced cell proliferation,migration and invasion of DLBCL cells.TBL1XR1 gene mutation significantly increased the expression of N-cadherin protein,while the expression of E-cadherin protein decreased.Conclusion:TBL1XR1 mutation plays a role in promoting tumor cell proliferation,migration and invasion in DLBCL.TBL1XR1 could be considered as a potential target for DLBCL therapy in future research.

关 键 词：弥漫大B细胞淋巴瘤 TBL1XR1突变 上皮间质转化 

分 类 号：R733.1[医药卫生—肿瘤]