一株乌拉尔甘草内生真菌的分离鉴定及其抑菌活性评价  

Isolation,Identification and Antibacterial Activity Evaluation of an Endophytic Fungus from Glycyrrhiza uralensis Fisch

作　　者：熊财智 曹金凤 赵江源[2] 李铭刚[2] 丁建海[1] XIONG Cai-zhi;CAO Jin-feng;ZHAO Jiang-yuan(Ningxia Normal University,Guyuan,Ningxia 756000;Yunnan Insti-tute of Microbiology,Yunnan University,Kunming,Yunnan 650091)

机构地区：[1]宁夏师范学院,宁夏固原756000 [2]云南大学云南省微生物研究所,云南昆明650091

出　　处：《安徽农业科学》2025年第5期149-152,共4页Journal of Anhui Agricultural Sciences

基　　金：宁夏重点研发计划项目(2023BEG03061);宁夏自然科学(2022AAC02049,2022AAC03297,2023AAC03355);宁夏高等学校科学研究项目(NYG2022073)。

摘　　要：采用组织块分离法,从野生乌拉尔甘草(Glycyrrhiza uralensis Fisch.)的根部分离得到一株内生真菌GG5-1,经分子生物学鉴定为Neocosmospora rubicola。通过生长速率法对油菜菌核病菌(Sclerotinia sclerotiorum)和葡萄灰霉病菌(Botrytis cinerea)进行抑菌活性评价,结果表明,乌拉尔甘草内生真菌GG5-1大米发酵提取物在0.1 mg/mL对油菜菌核病菌有强烈的抑制效果,抑制率为(97.28±0.77)%。An endophytic fungal strain GG5-1 was isolated from the roots of Glycyrrhiza uralensis by the method of tissue-block isolation and identified by molecular biology as Neocosmospora rubicola.Bacteriostatic experiments on Sclerotinia sclerotiorum and Botrytis cinerea were con-ducted by the growth rate method,the results showed that rice fermentation extract of endophytic fungus GG5-1 from G.uralensis had a strong inhibitory effect against Sclerotinia sclerotiorum at 0.1 mg/mL,with an inhibition rate of(97.28±0.77)%.

关 键 词：乌拉尔甘草 内生真菌 分离鉴定 抑菌活性 

分 类 号：Q93[生物学—微生物学]