Innovative genome editing in plants: a transposase and CRISPR combination approach  

作　　者：Hamza Sohail Iqra Noor Xuehao Chen Xiaodong Yang 

机构地区：[1]School of Horticulture and Landscape Architecture,Yangzhou University,Yangzhou 225009,China

出　　处：《Science China(Life Sciences)》2025年第3期893-894,共2页中国科学(生命科学英文版)

基　　金：supported by Jiangsu specially-appointed professor fund(337060046);the Yangzhou University Startup Fund(137012599).

摘　　要：Transgenic plants are extensively utilized in research,enabling scientists to explore gene functions in controlled environments,leading to crops with higher productivity and greater resistance to biotic and abiotic stressors(Oyebamiji et al.,2024).Traditional transgene integration techniques,such as particle bombardment and Agrobacterium T-DNA integration,often lead to unintended mutations and unpredictable insertion sites,emphasizing the necessity for developing targeted transgene integration(Ahuja and Fladung,2014).Current methods for targeted transgene integration,like homologous recombination(HR)and CRISPR-Cas,including their variants like prime editing and homology-directed repair(HDR),face challenges due to low integration efficiency and high deletion rates,hampering their practical application in crop genetic engineering(Dong and Ronald,2021;Tzfira and Citovsky,2006).

关 键 词：integration INSERTION CROPS 

分 类 号：Q943[生物学—植物学]