CXCL16 promotes proliferation of head and neck squamous cell carcinoma by regulating GPX1-mediated antioxidant levels  

作　　者：Ru HE Hongyi JIANG Chengchi ZHANG Yuan CHEN Wenshun LIU Xinyue DENG Xiaozheng ZHU Yunye LIU Chuanming ZHENG Yining ZHANG Chengying SHAO Yanting DUAN Jiajie XU 

机构地区：[1]Otolaryngology&Head and Neck Center,Cancer Center,Department of Head and Neck Surgery,Zhejiang Provincial People’s Hospital(Affiliated People’s Hospital),Hangzhou Medical College,Hangzhou 310014,China [2]College of Biotechnology and Bioengineering,Zhejiang University of Technology,Hangzhou 310014,China [3]Cancer Center,Department of Pathology,Zhejiang Provincial People’s Hospital(Affiliated People’s Hospital),Hangzhou Medical College,Hangzhou 310014,China [4]Department of Postgraduate Education,Jinzhou Medical University,Jinzhou 121000,China [5]The Second School of Clinical Medicine,Zhejiang Chinese Medical University,Hangzhou 310053,China [6]Zhejiang Key Laboratory of Precision Medicine Research on Head&Neck Cancer,Hangzhou 310014,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2025年第1期92-106,共15页浙江大学学报(英文版)B辑(生物医学与生物技术)

基　　金：supported by the Scientific Research Fund of the National Health Commission-Zhejiang Provincial Health Major Science and Technology Plan Project(No.WKJ-ZJ-2415);the Key Research and Development Program of Zhejiang Province(No.2024C03166);the Traditional Chinese Medicine Science and Technology Project of Zhejiang Province(No.2022ZB020);the Zhejiang Provincial Natural Science Foundation of China(No.LY21H160049).

摘　　要：Numerous studies have demonstrated that the high expression of CXC motif chemokine ligand 16(CXCL16)in cancer correlates with poor prognosis,as well as tumor cell proliferation,migration,and invasion.While CXCL16 can serve as a tumor biomarker,the underlying mechanism in modulating head and neck squamous cell carcinoma(HNSCC)remains unclear.In this study,the aimed was to investigate the CXCL16 expression in HNSCC and to uncover the potential underlying mechanism.Hereby,we determined the high expression of CXCL16 in The Cancer Genome Atlas(TCGA)database,as well as in tissue samples from patients with HNSCC at our central hospital and from HNSCC cell lines.The results showed that CXCL16 knockdown inhibited the proliferation,migration,and invasion of HNSCC cells.Mechanistically,transcriptome sequencing revealed that CXCL16 may affect HNSCC cell growth by regulating the antioxidant pathway of glutathione peroxidase 1(GPX1).The reactive oxygen species(ROS)levels were elevated in small interfering CXCL16(si-CXCL16)cells,which may contribute to the inhibition of cell proliferation,migration,and invasion.Moreover,treatment of cells with the GPX1 inhibitor eldecalcitol(ED-71)revealed that HNSCC cell growth was significantly inhibited in the synergistic group of si-CXCL16 and GPX1 inhibitor compared to the si-CXCL16 group.In conclusion,CXCL16 contributed to the development of HNSCC cells by modulating the GPX1-mediated antioxidant pathway.Thus,targeting cellular CXCL16 expression seems to be a promising strategy for treating HNSCC.

关 键 词：Antioxidant pathway CXC motif chemokine ligand 16(CXCL16) Glutathione peroxidase 1(GPX1) Head and neck squamous cell carcinoma(HNSCC) 

分 类 号：R73[医药卫生—肿瘤]