SLC1A3调控PI3K/AKT通路影响前列腺癌转移  

Effect of SLC1A3 on the malignant metastasis of prostate cancer by regulating the PI3K/AKT pathway

作　　者：韩晖 孙飞宇 HAN Hui;SUN Feiyu(Department of Urology,Shanxi Provincial Cancer Hospital/Cancer Hospital of Chinese Academy of Medical Sciences Shanxi Hospital/Cancer Hospital Affiliated to Shanxi Medical University,Taiyuan 030013,China)

机构地区：[1]山西省肿瘤医院/中国医学科学院肿瘤医院山西医院/山西医科大学附属肿瘤医院泌尿外科,山西太原030013

出　　处：《生物技术》2025年第1期74-78,134,共6页Biotechnology

基　　金：山西省基础研究计划(自由探索类)青年科学研究项目(202303021212345)。

摘　　要：[目的]探索SLC1A3在前列腺癌中的表达以及对前列腺癌DU145细胞恶性转移的影响。[方法]蛋白免疫印迹分析前列腺癌细胞与前列腺正常细胞中SLC1A3的表达;将人前列腺癌DU145细胞分为3个实验组:siRNA NC组、siRNA SLC1A3组与LY294002组。成球实验法检测前列腺癌DU145细胞的生长活性;细胞划痕实验分析前列腺癌DU145细胞的迁移能力;蛋白免疫印迹实验检测DU145细胞中PI3K/AKT蛋白的表达;TUNEL实验检测前列腺癌DU145细胞的凋亡率。[结果]与前列腺正常细胞相比,前列腺癌细胞中SLC1A3的表达上调(0.31±0.05 vs 0.82±0.07,P<0.05)。与siRNA NC组相比,siRNA SLC1A3和LY294002组的前列腺癌DU145细胞生长活性减弱(P<0.05);siRNA SLC1A3和LY294002组的前列腺癌DU145细胞迁移数量下降(P<0.05);siRNA SLC1A3和LY294002组的前列腺癌DU145细胞凋亡率增加(P<0.05);siRNA SLC1A3组的前列腺癌DU145细胞的PI3K/AKT蛋白表达降低(0.79±0.09 vs 0.29±0.03 vs 0.26±0.05;0.75±0.21 vs 0.22±0.05 vs 0.19±0.07,P<0.05)。[结论]SLC1A3在前列腺癌细胞中表达上调,抑制SLC1A3表达后,前列腺癌DU145细胞的生长活性以及迁移能力降低,凋亡率增加,这一过程与SLC1A3调节PI3K/AKT途径蛋白的表达相关。[Objective]To explore the expression of SLC1A3 in prostate cancer and its effect on the malignant metastasis of prostate cancer DU145 cells.[Method]Western Blot was used to analyze the expression of SLC1A3 in prostate cancer cells and normal prostate cells.Human prostate cancer DU145 cells were divided into three experimental groups:siRNA NC group,siRNA SLC1A3 group and LY294002 group.The growth activity of DU145 cells was detected by sphere formation assay.The migration ability of DU145 cells was analyzed by cell scratch test.The expression of PI3K/AKT protein in DU145 cells was detected by Western Blot.The apoptosis rate of DU145 cells was detected by TUNEL assay.[Result]SLC1A3 expression was upregulated in prostate cancer cells compared to prostate normal cells(0.31±0.05 vs 0.82±0.07,P<0.05).Compared with the siRNA NC group,the growth activity of DU145 cells in the siRNA SLC1A3 and LY294002 groups was decreased(P<0.05).The migration number of DU145 cells in the siRNA SLC1A3 and LY294002 groups was decreased(P<0.05).The apoptosis rate of DU145 cells in the siRNA SLC1A3 and LY294002 groups was increased(P<0.05).The expression of PI3K/AKT protein in prostate cancer DU145 cells in the siRNA SLC1A3 group was decreased(0.79±0.09 vs 0.29±0.03 vs 0.26±0.05;0.75±0.21 vs 0.22±0.05 vs 0.19±0.07,P<0.05).[Conclusion]The expression of SLC1A3 is up-regulated in prostate cancer cells.After inhibition of SLC1A3 expression,the growth activity and migration ability of prostate cancer DU145 cells are decreased,and the apoptosis rate is increased.This process is related to the regulation of SLC1A3 on the expression of PI3K/AKT pathway proteins.

关 键 词：SLC1A3 增殖 PI3K 迁移 前列腺癌 AKT 凋亡 DU145 

分 类 号：R737.2[医药卫生—肿瘤]