SPON1通过Vimentin/E-cadherin通路促进结直肠癌细胞转移  

SPON1 promoted the metastasis of colorectal cancer cells through the Vimentin/E-cadherin pathway

作　　者：杨斌 韩锦胜[1] 夏炳辉 YANG Bin;HAN Jinsheng;XIA Binghui(Department of General Surgery,Department of Orthopedics,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine,Cangzhou 061001,China)

机构地区：[1]河北省沧州中西医结合医院骨科院区综合外科,河北沧州061001

出　　处：《生物技术》2025年第1期79-83,共5页Biotechnology

基　　金：河北省医学科学研究课题(20211362)。

摘　　要：[目的]探讨底板反应蛋白1(SPON1)介导波形蛋白(Vimentin)/上皮型钙粘素(E-cadherin)信号通路对于结直肠癌细胞转移的影响。[方法]采集2021年4月-2022年7月手术的78例结直肠癌患者的癌组织及癌旁组织制作石蜡标本,免疫组化法检测其SPON1蛋白水平。随机将SW620细胞分为空白组、阴性对照组和SPON1敲低组,将阴性对照质粒、SPON1敲低质粒分别转入阴性对照组和SPON1敲低组的SW620细胞中,构建细胞模型,检测EMT相关蛋白Vimentin、E-cadherin蛋白表达。[结果]结直肠癌组织SPON1蛋白阳性表达率85.90%(67/78)高于癌旁组织16.67%(13/78),其SPON1蛋白高表达率84.62%(66/78)高于癌旁组织19.23%(15/78)。与空白组、阴性对照组的SW620细胞比较,SPON1敲低组的SW620细胞侵袭数量与迁移率降低(89.26±8.19 vs 89.26±8.19 vs 22.38±5.11/个;75.23%±6.28%vs 78.15%±11.29%vs 26.73%±3.16%);SPON1敲低组的SW620细胞中SPON1 mRNA、SPON1和Vimentin蛋白水平下降(P<0.05),E-cadherin蛋白水平增加(P<0.05)。[结论]SPON1在结肠癌中表达上调,敲低SPON1的表达能下调Vimentin表达,上调E-cadherin表达,抑制结肠癌细胞的转移。[Objective]To investigate the effect of plate-reactive protein 1(SPON1)on the metastasis of colorectal cancer cells through Vimentin/E-cadherin signaling pathway.[Method]Seventy-eight patients with colorectal cancer who underwent surgical treatment from April 2021 to July 2022 were collected,and their cancer tissues and adjacent tissues were taken for paraffin preparation.SPON1 protein levels in the cancer tissues and adjacent tissues were detected by immunohistochemistry.SW620 cells were randomly divided into blank group,negative control group and SPON1 knockdown group.The negative control plasmid and SPON1 knockdown plasmid were transferred into SW620 cells of negative control group and SPON1 knockdown group to construct a cell model,and the expressions of EMT-related proteins Vimentin and E-cadherin were detected.[Result]The positive expression rate of SPON1 protein in colorectal cancer tissues was 85.90%(67/78),which was higher than that in adjacent tissues 16.67%(13/78),and the high expression rate of SPON1 protein was 84.62%(66/78),which was higher than that in adjacent tissues 19.23%(15/78)(P<0.05).Compared with the blank group and the negative control group,the invasion ability and migration rate of SW620 cells in the SPON1 knockdown group were decreased(89.26±8.19 vs 89.26±8.19 vs 22.38±5.11/cell;75.23%±6.28%vs 78.15%±11.29%vs 26.73%±3.16%);The levels of SPON1 mRNA,SPON1 and Vimentin protein in SW620 cells of SPON1 knockdown group were decreased(P<0.05),increased E-cadherin protein level(P<0.05).[Conclusion]The expression of SPON1 is up-regulated in colon cancer.Knockdown of SPON1 can down-regulate the expression of Vimentin,up-regulate the expression of E-cadherin,and inhibit the metastasis of colon cancer cells.

关 键 词：结直肠癌 底板反应蛋白1 波形蛋白 上皮型钙粘素 上皮间充质转化 SW620细胞 转移 侵袭 

分 类 号：R735.5[医药卫生—肿瘤]