S100A12调控RAGE影响宫颈癌细胞增殖、迁移和凋亡  

Effects of S100A12 on proliferation,migration and apoptosis of cervical cancer cells by regulating RAGE

作　　者：姜文静[1] 苏根娣 郑荣荣[1] 季兰萍 张林艳 JIANG Wenjing;SU Gendi;ZHENG Rongrong;JI Lanping;ZHANG Linyan(Department of Obstetrics and Gynecology,Jianhu County People′s Hospital,Yancheng 224700,China)

机构地区：[1]建湖县人民医院妇产科,江苏盐城224700

出　　处：《生物技术》2025年第1期84-89,共6页Biotechnology

基　　金：江苏医药职业学院校地协同创新研究项目(20239210)。

摘　　要：[目的]探究S100A12、RAGE在宫颈癌细胞增殖、迁移和凋亡中的作用。[方法]采集2022年4月-2023年5月宫颈癌病例癌组织及癌旁组织的石蜡标本,用免疫组化法检测S100A12的表达。随机将SiHa细胞分为空白组(无转染)、阴性对照组(转染空白质粒)和敲低组(转染siRNA),构建相应细胞模型。免疫组化检测S100A12在宫颈癌组织和癌旁组织的表达情况;实时荧光定量PCR检测S100A12 mRNA与RAGE mRNA水平;Western Blot检测PCNA、S100A12的蛋白表达水平;通过MTT法以及细胞集落形成实验检测细胞的生长情况,通过Transwell实验检测细胞迁移情况;流式细胞术检测细胞凋亡情况。[结果]S100A12在宫颈癌组织中蛋白表达水平高于癌旁组织(82.47%±0.35%vs 11.34%±0.17%,P<0.05)。敲低组细胞中S100A12、RAGE的mRNA和蛋白表达均低于空白组与阴性对照组(1.16±0.12 vs 1.13±0.16 vs 0.17±0.08;2.57±0.13 vs 2.67±0.13 vs 0.65±0.05;P<0.05)。敲低组细胞的增殖能力、集落形成数量和迁移能力均低于空白组、阴性对照组(P<0.05)。敲低组细胞的凋亡率高于空白组、阴性对照组(1.16%±0.09%vs 1.78%±0.03%vs 11.53%±0.09%;P<0.05)。[结论]宫颈癌组织中RAGE与S100A12表达量显著升高,下调S100A12后,癌细胞的增殖、迁移能力减弱,凋亡率增加,并且RAGE表达受到抑制。因此,S100A12对宫颈癌细胞的抑制作用与调控RAGE相关。[Objective]To explore the role of S100A12 and RAGE in the migration,proliferation and apoptosis of cervical cancer cells.[Method]Paraffin specimens of cervical cancer tissues and adjacent tissues were collected from April,2022 to May,2023 in our hospital,and the expression of S100A12 was checked by immunohistochemistry.SiHa cells were randomly divided into negative control group(transfected with blank plasmid),blank group(no transfection)and knockdown group(transfected with siRNA),and the corresponding cell models were constructed.The expression of S100A12 checked by immunohistochemistry.The levels of S100A12 mRNA and RAGE mRNA were checked by real-time PCR.The protein expression of S100A12 and PCNA was checked by Western Blot.The cell growth was checked by MTT assay and cell colony formation assay.The cell migration was detected by Transwell assay cell apoptosis was checked by flow cytometry.[Result]In cervical cancer tissues,the protein expression level of S100A12 was higher than adjacent tissues(82.47%±0.35%vs 11.34%±0.17%,P<0.05).The mRNA and protein expressions of S100A12 and RAGE in knockdown group were lower than blank and negative control group(1.16±0.12 vs 1.13±0.16 vs 0.17±0.08;2.57±0.13 vs 2.67±0.13 vs 0.65±0.05;P<0.05).The proliferation,colony formation and migration ability of the knockdown group were significantly lower than those of the blank group and the negative control group(P<0.05).The apoptosis rate of knockdown group was higher than that of blank group and negative control group(1.16%±0.09%vs 1.78%±0.03%vs 11.53%±0.09%;P<0.05).[Conclusion]The expressions of RAGE and S100A12 are significantly increased in cervical cancer tissues.After S100A12 knockdown,the proliferation and migration of cancer cells are weakened,the apoptosis rate is increased,and the expression of RAGE is inhibited.Therefore,the inhibitory effect of S100A12 on cervical cancer cells is related to the regulation of RAGE.

关 键 词：S100A12 RAGE 宫颈癌 SIHA细胞 迁移 凋亡 增殖 免疫组化 

分 类 号：R737.3[医药卫生—肿瘤]