肝郁气滞型三阴性乳腺癌口腔菌群的相关性研究  

Correlation Study on Oral Flora of Triple Negative Breast Cancer of Liver Depression and Qi Stagnation Type

作　　者：孙梦莹 张爱琴[2] 陈卓[2] SUN Mengying;ZHANG Aiqin;CHEN Zhuo(Zhejiang Chinese Medical University,Zhejiang Hangzhou 310053,China;Zhejiang Cancer Hospital,Zhejiang Hangzhou 310022,China)

机构地区：[1]浙江中医药大学,浙江杭州310053 [2]浙江省肿瘤医院,浙江杭州310022

出　　处：《中医药临床杂志》2025年第3期520-526,共7页Clinical Journal of Traditional Chinese Medicine

基　　金：2022年度浙江省中医药优秀青年人才基金项目(2022ZQ015)。

摘　　要：目的:研究肝郁气滞型三阴性乳腺癌(triple negative breast cancer,TNBC)患者口腔菌群的多样性与差异性,探讨口腔菌群与肝郁气滞型TNBC的相关性。方法:根据纳排标准纳入肝郁气滞型三阴性乳腺癌患者8例作为患者组,健康志愿者10例作为正常组,分别收集唾液标本,采用16S rDNA测序技术检测2组人群口腔菌群的丰度和分布情况,通过组间差异分析寻找组间具有显著性差异的菌属。结果:患者组与正常组的口腔菌群物种多样性显著,且2组口腔菌群分布存在显著差异(P<0.05)。其中患者组较正常组增多的物种有密螺旋体属(Treponema)、普雷沃氏菌属(Prevotella)、噬二氧化碳细胞菌属(Capnocytophaga)、坦纳菌属(Tannerella)、考拉杆菌属(Phascolarctobacterium)、金氏菌属(Kingella)、丛毛单胞菌属(Comamonas)、螺旋体科(Spirochaetaceae)等;较正常组减少的物种有韦荣氏球菌(Veillonella)、链球菌属(Streptococcus) 、己酸菌属(Caproiciproducens)、气单胞菌属(Aeromonas)等。结论:失调的口腔菌群及其细菌产物在三阴性乳腺癌的发生发展和转移中起着重要作用,同时特定菌群对中医证候的形成与变化也有一定影响。口腔菌群可作为TNBC早期诊断、预后判断以及中医证候客观化的生物标志物,对于提高TNBC的疗效、靶向干预相关不良反应、改善生活质量及预后有着重要意义。Objective:To study the diversity and difference of oral microbiota in patients with triple negative breast cancer(TNBC)of liver depression and qi stagnation,and discussed the correlation between oral flora and TNBC of liver stagnation and qi stagnation.Methods:According to the inclusion and exclusion criteria,8 patients with TNBC of liver depression and qi stagnation type were included as the patient group,and 10 healthy volunteers were included as the normal group.Saliva samples were collected respectively.The abundance and distribution of oral microbiota in the two groups were detected by 16S rDNA sequencing technology,and the genus with significant difference(P<0.05)be-tween the two groups was found by difference analysis.Results:The species richness of oral microbiota in patients with TNBC and healthy people was significant,and there was a significant difference in the distribution of oral microbiota between the two groups(P<0.05).Among them,Treponema,Prevotella,Capnocytophaga,Tannerella,Phascolarc-tobacterium,Kingella,Comamonas,Spirochaetaceae and so on were increased in the patient group compared with the normal group.And the species less than the normal group included Veillonella,Streptococcus,Caproiciproducens,Aeromonas and so on.Conclusion:Dysregulated oral microbiota and its bacterial products play an important role in the occurrence,development and metastasis of TNBC.At the same time,specific microbiota also has a certain influence on the formation and change of traditional Chinese medicine(TCM)syndromes.Oral microbiota can be used as a bio-marker for early diagnosis,prognosis judgment and TCM syndrome objectification of TNBC,which is of great signifi-cance for improving the efficacy of TNBC,targeted intervention related adverse reactions and improving quality of life and prognosis.

关 键 词：三阴性乳腺癌 肝郁气滞 中医证型 口腔菌群 16S rDNA 

分 类 号：R742.4[医药卫生—神经病学与精神病学]