参苓白术散改良方发酵工艺及对GES-1细胞酒精性损伤的干预作用研究  

Study on Fermentation Technology of Shenling Baizhu Powder(参苓白术散)and Its Intervention Effect on Alcohol Injury of GES-1 Cells

作　　者：刘红宁[1] 郑凯麒 陈丽华[1] 姜佳恩 周权 LIU Hongning;ZHENG Kaiqi;CHEN Lihua;JIANG Jiaen;ZHOU Quan(Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi,China)

机构地区：[1]江西中医药大学,江西南昌330004

出　　处：《中华中医药学刊》2025年第3期6-12,I0001,共8页Chinese Archives of Traditional Chinese Medicine

基　　金：江西省科技计划“揭榜挂帅”关键技术类项目(20212AAF01005);江西中医药大学校级科技创新团队发展计划项目(CXTD-22004)。

摘　　要：目的研究阿氏肠杆菌和蛹虫草真菌发酵参苓白术散改良方最佳发酵条件,并在细胞层面评价发酵液的安全性和对酒精性胃损伤治疗的有效性。方法实验检测经不同条件发酵后的参苓白术散改良方发酵液中功能成分的含量,并进行综合评价,在此基础上利用单因素实验对发酵条件进行优化;以酒精作用24 h建立GES-1细胞酒精性损伤模型,考察参苓白术散改良方水提液(0.938 mg/g)和发酵液在低、中、高剂量(0.469、0.938、1.876 mg/g)对人胃黏膜上皮细胞株GES-1细胞酒精性损伤基础凋亡的干预作用。结果阿氏肠杆菌发酵参苓白术散改良方的最佳发酵工艺为pH值5,接种量1%,发酵时间1 d。蛹虫草真菌发酵参苓白术散改良方的最佳发酵工艺为pH值6,接种量3%,发酵时间3 d。多菌种发酵最佳接种顺序为先接种阿氏肠杆菌。最佳发酵工艺下人参皂苷Re、橙皮苷、总黄酮和总多糖含量均得到显著性提高;参苓白术散改良方发酵液作用于GES-1细胞48 h时使细胞增殖活力提高,且中、高剂量组发酵液能降低GES-1细胞酒精性损伤的总凋亡率。结论参苓白术散改良方发酵后其成分影响较大,含量有明显变化,发酵工艺可行,且对GES-1细胞有增殖作用,同时能降低GES-1细胞酒精性损伤的总凋亡率。Objective To study the optimal fermentation conditions of Enterobacter albieri and Cordyceps pupae for Shenling Baizhu Powder(参苓白术散),and evaluate the safety and efficacy of the fermentation solution on alcoholic gastric injury at cell level.Methods In this experiment,the contents of functional components in fermentation broth of the modified formula of Shenling Baizhu Powder after fermentation under different conditions were measured and comprehensively evaluated.The alcoholic gastric injury model of GES-1 cells was established after 24 h of alcohol intervention.The intervention effects of water extract(0.938 mg/g)and fermentation broth of the modified formula of Shenling Baizhu Powder at low,medium and high doses(0.469 mg/g,0.938 mg/g,1.876 mg/g)on basic apoptosis of GES-1 cells were investigated.Results The optimal fermentation process of Enterobacter asburiae for Shenling Baizhu Powder was pH value 5,inoculation amount 1%and fermentation time 1 day.The opti-mal fermentation process of Shenling Baizhu Powder was pH 6,inoculation amount 3%and fermentation time 3 days.The best in-oculation sequence for multistrain fermentation was to vaccinate Enterobacter asburiae first.The contents of ginsenoside Re,hes-peridin,total flavonoids and total polysaccharides were significantly increased under the optimal fermentation process.The fer-mentation solution of Shenling Baizhu Powder improved GES-1 cells at 48 h,and the fermentation solution of medium and high dose groups could reduce the total apoptosis rate of GES-1 cells.Conclusion After fermentation,the composition of the modified formula of Shenling Baizhu Powder had a great influence,the content changed obviously and the fermentation process was feasi-ble.It had a proliferation effect on GES-1 cells,and at the same time it could reduce the overall apoptosis rate of GES-1 cells.

关 键 词：微生物 发酵 GES-1细胞 酒精性胃损伤 

分 类 号：R284.1[医药卫生—中药学]