宫颈癌肿瘤组织miR-222-3p、SLFN11表达与临床病理特征及预后的相关性  

Correlation between miR-222-3p and SLFN11 expressions in cervical cancer tumor tissues and clinicopathological features and prognosis

作　　者：张朋 曾小艳 周晓莉 汪黎明[2] 张雅君 Zhang Peng;Zeng Xiaoyan;Zhou Xiaoli;Wang Liming;Zhang Yajun(Department of Obstetrics and Gynecology,General Hospital of Central Theater Command of PLA,Hubei,Wuhan 433000,China;不详)

机构地区：[1]中国人民解放军中部战区总医院妇产科,武汉433000 [2]湖北省妇幼保健院妇产科,武汉430070 [3]湖北文理学院附属襄阳市中心医院妇产科,襄阳441021

出　　处：《疑难病杂志》2025年第3期296-302,共7页Chinese Journal of Difficult and Complicated Cases

基　　金：湖北省自然科学基金资助项目(2020HBA256)。

摘　　要：目的研究宫颈癌肿瘤组织miR-222-3p、Schlafen家族成员11(SLFN11)表达与临床病理特征及预后的相关性。方法纳入2019年1月-2021年6月中国人民解放军中部战区总医院妇产科治疗的宫颈癌患者103例作为宫颈癌组,宫颈良性疾病患者57例作为宫颈良性病组。实时荧光定量聚合酶链式反应检测2组患者miR-222-3p、SLFN11表达;分析宫颈癌患者不同临床病理特征中肿瘤组织miR-222-3p及SLFN11表达的差异;相关性分析采用Spearman秩相关;预测宫颈癌预后不良效能采用ROC曲线分析并用DeLong法比较,危险因素采用多因素Cox回归分析。生存分析采用K-M法分析并采用Log Rank比较。结果宫颈癌组肿瘤组织miR-222-3p表达高于癌旁组织和宫颈良性病组,SLFN11表达低于癌旁组织和宫颈良性病组(F/P=945.371/<0.001、226.816/<0.001);CTC阳性、Ki-67≥70%、T分期T3~4、N分期N1、远处转移M1及TNM分期Ⅲ~Ⅳ期患者miR-222-3p表达高于CTC阴性、Ki-67<70%、T分期T1~2、N分期Nx~N0、无远处转移及TNM分期Ⅰ~Ⅱ期患者(t/P=3.263/0.002、4.208/<0.001、4.182/<0.001、3.342/0.001、5.391/<0.001、3.298/<0.001),SLFN11表达低于CTC阴性、Ki-67<70%、T分期T1~2、N分期Nx~N0、无远处转移及TNM分期Ⅰ~Ⅱ期患者(t/P=5.140/<0.001、5.706/<0.001、5.984/<0.001、7.701/<0.001、6.704/<0.001、7.859/<0.001)。宫颈癌组肿瘤组织miR-222-3p表达与CTC、Ki-67、T分期、N分期、远处转移、TNM分期呈正相关(r s/P=0.683/0.035、0.704/0.009、0.626/0.021、0.651/0.029、0.627/0.023、0.649/0.015),SLFN11表达与CTC、Ki-67、T分期、N分期、远处转移、TNM分期呈负相关(r s/P=-0.624/0.037、-0.651/0.025、-0.677/0.032、-0.632/0.019、-0.648/0.007、-0.675/0.023)。miR-222-3p、SLFN11及二者联合预测宫颈癌患者预后不良的曲线下面积(AUC)分别为0.674、0.652、0.883,二者联合优于各自单独预测效能(Z/P=8.143/<0.001、10.561/<0.001)。CTC阳性、Ki-67≥70%、T分期T3~4、N分期N1、有远处�Objective To investigate the correlation between the expression of miR-222-3p and SLFN11 in cervical cancer tumor tissue and clinical pathological characteristics and prognosis.Methods A total of 103 patients with cervical cancer treated in the General Hospital of the Central Theater Command of the Chinese People's Liberation Army from January 2019 to June 2021 were divided into the cervical cancer group and 57 patients with benign cervical diseases as the control group.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of miR-222-3p and SLFN11 in the two groups of patients;the differences in the expression of miR-222-3p and SLFN11 in tumor tissues of patients with different clinical pathological characteristics of cervical cancer were analyzed;Correlation analysis was performed using Spearman rank correlation,poor prognostic efficacy of cervical cancer was analyzed using ROC curve analysis and compared using DeLong method,risk factors were analyzed using multivariate Cox regression analysis,and survival analysis was performed using K-M method and Log Rank comparison.Results The expression of miR-222-3p in tumor tissue of cervical cancer group was higher than that in adjacent tissues and control group,while the expression of SLFN11 in adjacent tissues and control group was lower(F/P=945.371/<0.001,226.816/<0.001).The expression of miR-222-3p in patients with positive CTC,Ki-67≥70%,T stage T3-4,N stage N1,distant metastasis M1 and TNM stageⅢ-Ⅳwas higher than that in patients with negative CTC,Ki-67<70%,T stage T1-2,N stage Nx-N0,no distant metastasis and TNM stageⅠ-Ⅱ(t/P=3.263/0.002,4.208/<0.001,4.182/<0.001,3.342/0.001,5.391/<0.001,3.298/<0.001),SLFN11 expression was lower than that in patients with negative CTC,Ki-67<70%,T stage T1-2,N stage Nx-N0,no distant metastasis and TNM stageⅠ-Ⅱ(t/P=5.140/<0.001,5.706/<0.001,5.984/<0.001,7.701/<0.001,6.704/<0.001,7.859/<0.001)In the cervical cancer group,the expression of miR-222-3p in tumor tissue was positively

关 键 词：宫颈癌 微小RNA-222-3p Schlafen家族成员11 预后 

分 类 号：R737.33[医药卫生—肿瘤]