原发性开角型青光眼患者血清miR-21、miR-15b表达水平与视神经损伤程度和炎性反应的相关性  

Study on the correlation between peripheral blood miR-21 and miR-15b expression levels and the degree of optic nerve damage and inflammatory response in patients with primary open-angle glaucoma

作　　者：杨阳 万玮[2] 王雅琪 纪开宝 张雯茜[3] 陈婷 Yang Yang;Wan Wei;Wang Yaqi;Ji Kaibao;Zhang Wenqian;Chen Ting(Department of Ophthalmology,Renmin Hospital of Wuhan University,Hubei,Wuhan 430060,China;不详)

机构地区：[1]武汉大学人民医院眼二科,430060 [2]武汉大学人民医院眼三科,430060 [3]武汉大学人民医院眼一科,430060

出　　处：《疑难病杂志》2025年第3期343-348,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：湖北省重点实验室开放项目(2023KFH015、2023KFH019)。

摘　　要：目的探究原发性开角型青光眼(POAG)患者血清微小RNA-21(miR-21)、微小RNA-15b(miR-15b)表达水平与视神经损伤程度和炎性反应的相关性。方法选取2023年3月-2024年3月武汉大学人民医院眼科收治的POAG患者112例作为病例组,根据平均缺损值(MD)评估视神经损害程度并将患者分为轻度亚组(n=47)、中度亚组(n=35)、重度亚组(n=30);另选取同期健康体检者100例作为健康对照组。采用实时荧光定量PCR法检测血清miR-21、miR-15b表达水平;Pearson相关性分析POAG患者血清miR-21、miR-15b水平与血清炎性指标的相关性;ROC曲线分析血清miR-21、miR-15b对视神经损伤程度的评估价值。结果病例组低密度脂蛋白胆固醇(LDL-C)、miR-21水平高于健康对照组,高密度脂蛋白胆固醇(HDL-C)、miR-15b水平显著低于健康对照组(t/P=3.902/<0.001、9.209/<0.001、4.913/<0.001、17.764/<0.001)。血清miR-21水平比较,重度亚组>中度亚组>轻度亚组,miR-15b水平比较,重度亚组<中度亚组<轻度亚组(F/P=41.478/<0.001、15.411/<0.001)。病例组血清白介素-1β(IL-1β)、IL-18水平高于健康对照组,而血清IL-6、IL-12水平低于健康对照组(t/P=19.781/<0.001、28.338/<0.001、20.271/<0.001、16.786/<0.001)。患者血清miR-21水平与炎性指标IL-1β、IL-18呈正相关,与IL-6、IL-12呈负相关(r/P=0.572/<0.001、0.632/<0.001、-0.518/<0.001、-0.498/<0.001),血清miR-15b水平与炎性指标IL-1β、IL-18呈负相关,与IL-6、IL-12呈正相关(r/P=-0.621/<0.001、-0.563/<0.001、0.598/<0.001、0.612/<0.001)。血清miR-21、miR-15b及二者联合评估POAG患者视神经损伤重度的曲线下面积(AUC)分别为0.841、0.757、0.898,二者联合优于血清miR-21、miR-15b各自单独检测(Z/P=2.900/0.004、2.743/0.006)。结论POAG患者血清miR-21水平明显升高,而血清miR-15b水平明显降低,与视神经损伤程度和炎性反应有显著相关性,二者联合检测对POAG患者视神经损伤程度有更高的评估价值。Objective To investigate the correlation between the expression levels of peripheral blood microRNA-21(miR-21)and microRNA-15b(miR-15b)in patients with primary open-angle glaucoma(POAG)and the degree of optic nerve damage and inflammatory response.Methods A total of 112 POAG patients admitted to Department of Ophthalmology,Wuhan University People's Hospital from March 2023 to March 2024 were included as the case group.Patients were assigned into mild subgroup(n=47),moderate subgroup(n=35),and severe subgroup(n=30)based on the degree of optic nerve damage assessed by the mean defect value(MD).Another 100 healthy individuals who underwent physical checkups were selected as the control group.Real-time fluorescence quantitative PCR was applied to detect the expression levels of miR-21 and miR-15b in peripheral blood.Pearson correlation was applied to analyze the correlation between peripheral blood miR-21,miR-15b levels and inflammatory index in POAG patients.ROC curve was applied to analyze the evaluation value of peripheral blood miR-21 and miR-15b in assessing the degree of optic nerve damage.Results The levels of low-density lipoprotein cholesterol(LDL-C)and peripheral blood miR-21 in the case group were obviously higher than those in the control group,while the levels of high-density lipoprotein cholesterol(HDL-C)and peripheral blood miR-15b were obviously lower than those in the control group(t/P=3.902/<0.001,9.209/<0.001,4.913/<0.001,17.764/<0.001).The level of miR-21 in peripheral blood of the severe subgroup was obviously higher than that of the mild and moderate subgroups,while the level of miR-15b was obviously lower than that of the mild and moderate groups(F/P=41.478/<0.001,15.411/<0.001).The levels of inflammatory markers interleukin-1β(IL-1β)and interleukin-18(IL-18)in the case group were obviously higher than those in the control group,while the levels of interleukin-6(IL-6)and interleukin-12(IL-12)in peripheral blood were obviously lower than those in the control group(t/P=19.781/<0.001,28.338/<0.0

关 键 词：原发性开角型青光眼 微小RNA-21 微小RNA-15b 视神经损伤程度 炎性反应 

分 类 号：R775.2[医药卫生—眼科]