整合素连接激酶ILK在BRAF突变型甲状腺癌中的表达及临床意义  

Expression and clinical significance of integrin linked kinase ILK in BRAF mutant thyroid cancer

作　　者：杨萌萌 陈红跃[1] 王心雨 YANG Mengmeng;CHEN Hongyue;WANG Xinyu(Department of Thyroid and Breast Surgery,Henan Provincial Hospital of Traditional Chinese Medicine,Henan Zhengzhou 450003,China)

机构地区：[1]河南省中医院甲状腺乳腺外科,河南郑州450003

出　　处：《现代肿瘤医学》2025年第4期614-619,共6页Journal of Modern Oncology

基　　金：河南省中医药科学研究专项课题(编号:2024ZY1016)。

摘　　要：目的:探究BRAF突变与未突变型甲状腺癌患者癌灶组织中ILK蛋白的表达差异,探究ILK蛋白的临床意义。方法:收集2020年12月至2023年06月在我院行甲状腺切除术,术后诊断为甲状腺乳头状癌的46例患者的基本信息,统计所有入组患者BRAF基因突变和性别、年龄、原发灶直径、癌灶分布情况、彩超TI-RADS分级、癌症TNM及病理分期、原发灶是否侵及甲状腺外包膜与淋巴结、是否伴发桥本甲状腺炎等病理参数之间的差异性。对所有入组患者的原发癌灶切片后行免疫组织化学染色,以评估ILK蛋白的强弱。根据前一步检测结果,记录46例组织ILK蛋白强弱的分级,分析ILK与PTC各项临床病理参数的相关性。统计并分析ILK表达的强弱程度与PTC患者是否伴BRAF V600E基因突变的关系。结果:BRAF V600E基因突变与PTC多发癌灶、较高分期和分级、淋巴结和包膜外浸润呈正性相关(P<0.05),与患者性别、年龄、TI-RADS分级、原发灶直径、是否伴桥本甲状腺炎等无明显相关性(P>0.05)。ILK蛋白在上皮乳头状组织中呈现强弱不等的阳性表达,强阳性信号与肿瘤较大直径、多灶浸润、淋巴结转移及包膜外侵等恶性生物学行为关系密切(P<0.05)。BRAF突变型PTC患者原发癌灶中检出ILK蛋白强阳性率远远高于未突变患者组(P<0.05)。结论:ILK蛋白在BRAF突变型甲状腺癌中高表达,与肿瘤较大直径、多灶浸润、淋巴结转移及包膜外侵等恶性行为关系密切,参与BRAF基因突变诱导的癌症侵袭进程。Objective:To investigate the differential expression of ILK protein in cancer tissue between BRAF mutant and non mutant thyroid cancer patients,and to explore the clinical significance of ILK protein.Methods:Basic information of 46 patients diagnosed with papillary thyroid carcinoma after thyroidectomy in our hospital from December 2020 to June 2023 was collected.Differences in BRAF gene mutations and pathological parameters such as gender,age,primary lesion diameter,tumor distribution,TI-RADS grading by ultrasound,TNM and pathological staging of cancer,invasion of the thyroid capsule and lymph nodes by the primary lesion,and presence of Hashimoto's thyroiditis were statistically analyzed for all enrolled patients.Immunohistochemical staining was performed on the primary cancer slices of all enrolled patients to evaluate the strength of ILK protein.Based on the previous detection results,the grading of ILK protein strength in 46 tissues was recorded,and the correlation between ILK and various clinical pathological parameters of PTC was analyzed.Statistically analyze the relationship between the intensity of ILK expression and whether PTC patients have BRAF V600E gene mutations.Results:BRAF V600E gene mutation was positively correlated with PTC multiple cancer lesions,higher stage and grade,lymph node and extracapsular infiltration(P<0.05),but not with gender,age,TI-RADS grade,primary lesion diameter,or presence of Hashimoto's thyroiditis(P>0.05).ILK protein shows varying degrees of positive expression in epithelial papillary tissues,and strong positive signals are closely related to malignant biological behaviors such as larger tumor diameter,multifocal infiltration,lymph node metastasis,and extracapsular invasion(P<0.05).The strong positive rate of ILK protein detected in the primary cancer lesions of BRAF mutant PTC patients is much higher than that of non mutant patients(P<0.05).Conclusion:ILK protein is highly expressed in BRAF mutant thyroid cancer and is closely related to malignant behaviors such as larger

关 键 词：整合素连接激酶 甲状腺乳头状癌 BRAF突变 

分 类 号：R736.1[医药卫生—肿瘤]