黑参提取物防脱发作用研究  

Study on the anti-hair loss effect of Black Ginseng

作　　者：杨亭 周明娟[2] 刘瀚璘 徐梦迪 綦占文 綦特 刘金平[1] 王翠竹[1] 耿晓宇 Yang Ting;Zhou Mingjuan;Liu Hanlin;Xu Mengdi;Qi Zhanwen;Qi Te;Liu Jinping;Wang Cuizhu;Geng Xiaoyu(College of Pharmacy,Jilin University,Changchun 130021;College of Chemistry,Jilin University,Changchun 130000;Yanbian Hangongfang Health Products Co.Ltd.,Yanji 133000;College of Pharmacy,Tonghua Normal University,Tonghua 134002)

机构地区：[1]吉林大学药学院,长春130021 [2]吉林大学化学学院,长春130000 [3]延边韩工坊健康制品有限公司,延吉133000 [4]通化师范学院医药学院,通化134002

出　　处：《国际老年医学杂志》2025年第2期174-184,共11页International Journal of Geriatrics

基　　金：吉林省科技发展计划项目(20210502005ZP);吉林省产业自主创新能力专项(2020C038-3)。

摘　　要：目的建立雄激素源性C57BL/6小鼠脱发模型,观察黑参提取物对小鼠脱发的改善作用,并基于网络药理学和分子对接方法探究其作用机制。方法通过皮下注射丙酸睾酮建立小鼠脱发模型,设立不同剂量的黑参提取物组,并与米诺地尔进行对照。通过毛发生长情况、真皮厚度、毛囊形态与计数、血清激素水平及皮肤组织中血管内皮生长因子(VEGF)和转化生长因子-β(TGF-β)含量等指标,评价黑参提取物对脱发的改善作用。基于前期研究得到黑参提取物的化学成分,利用Swiss Target Prediction和Symmap数据库获取黑参化学成分的靶点,结合Malacard、DisGeNET及Genecards数据库中脱发相关靶点构建“黑参-化学成分-交集靶点-脱发”互作网络,筛选关键化学成分和靶点,进行分子对接分析。结果黑参提取物组小鼠毛发生长优于模型组,真皮厚度增加,血清睾酮及二氢睾酮含量降低,雌二醇含量升高,皮肤VEGF表达增加,TGF-β表达下降,均存在剂量依赖性。筛选出关键化学成分去氧齐墩果酸、人参皂苷Rb_(1)、人参皂苷Rg_(3)、人参皂苷Rh_(4)和菜油甾醇,及关键靶点INS、AR、VEGFA、PPARG、LEP和CASP3。分子对接结果表明关键成分与靶点间结合稳定。结论黑参提取物对雄激素引起的脱发具有改善作用,可能通过调控AR等靶点及MAPK等信号通路发挥作用,提供了进一步研究与开发的科学依据。Objective To establish an androgenic alopecia model in C57BL/6 mice,observe the improvement effect of Black Ginseng extract on alopecia,and explore its mechanism using network pharmacology and molecular docking methods.Methods An androgenic alopecia model was established by subcutaneous injection of testosterone propionate in mice.Different doses of Black Ginseng extract groups were set up and compared with Minoxidil.The improvement effect of Black Ginseng extract on androgenic alopecia was evaluated based on hair growth,dermal thickness,hair follicle morphology and count,serum hormone levels,and skin tissue VEGF and TGF-βcontent.Based on previous studies,the chemical constituents of Black Ginseng extract were identified.Swiss Target Prediction and Symmap databases were used to obtain targets of Black Ginseng s chemical constituents,combined with alopecia-related targets from Malacard,DisGeNET,and Genecards databases to construct a"Black Ginseng-chemical composition-intersection target-alopecia"interaction network,screening key chemical components and targets,followed by molecular docking analysis.Results Mice in the Black Ginseng extract groups showed better hair growth than the model group,with increased dermal thickness,decreased serum testosterone and dihydrotestosterone levels,increased estradiol levels,increased VEGF expression,and decreased TGF-βexpression in skin,all in a dose-dependent manner.Key chemical components Deoxyoleanolic acid,Ginsenoside Rb_(1),Ginsenoside Rg_(3),Ginsenoside Rh_(4),and Campesterol,as well as key targets INS,AR,VEGFA,PPARG,LEP,and CASP3,were identified.Molecular docking results showed stable binding between key components and targets.Conclusion Black Ginseng extract improves androgen-induced alopecia in mice,likely by regulating targets such as AR and pathways like MAPK.This provides a scientific basis for further research and development of Black Ginseng.

关 键 词：黑参 脱发 雄激素性脱发 网络药理学 分子对接 

分 类 号：R28[医药卫生—中药学]