低频超声联合纳米微泡对老年脑血栓患者治疗效果评价及血清标志物相互作用的网络构建  

Evaluation of the therapeutic effect of low-frequency ultrasound combined with nano-microbubbles on the treatment of elderly patients with cerebral thrombosis and the construction of serum marker interaction network

作　　者：陈姗姗 周晓东 Chen Shanshan;Zhou Xiaodong(Department of Ultrasound Diagnosis and Treatment,Xi'an International Medical Center Hospital,Xi'an 710100)

机构地区：[1]西安国际医学中心医院超声诊疗科,西安710100

出　　处：《国际老年医学杂志》2025年第2期201-206,共6页International Journal of Geriatrics

基　　金：陕西省重点研发计划项目(2023-YBSF-121);陕西省自然科学基础研究计划项目(2023-JC-QN-0912)。

摘　　要：目的探究低频超声联合纳米微泡治疗对老年脑血栓患者神经、运动功能和血清学标志物水平的影响,并基于血清学标志物构建蛋白-蛋白相互作用(PPI)网络。方法①选取2022年3月-2023年9月西安国际医学中心医院治疗的136例老年脑血栓患者作为研究对象,采用随机数字表法将患者分为观察组和对照组,各68例。两组均予以静脉溶栓治疗,观察组同时给予低频超声联合纳米微泡治疗。比较两组血清学标志物水平、凝血功能、神经和运动功能。②通过String数据库构建PPI网络。对PPI网络节点基因进行基因本体(GO)功能富集分析和京都基因和基因组百科全书(KEGG)富集分析。结果①治疗后,观察组的六分钟步行距离试验、Barthel指数、上肢Fugl-Meyer评分(FMA)评分、下肢FMA评分均高于对照组(P<0.05),观察组的美国国立卫生院脑卒中量表评分低于对照组(P<0.05)。治疗后,观察组的血清营养因子血管内皮生长因子(VEGF)、神经生长因子(NGF)、脑源性神经营养因子(BDNF)和神经营养蛋白3(NT-3)水平均高于对照组(P<0.05),血清S100钙结合蛋白β(S100β)水平低于对照组(P<0.05)。治疗后,两组的血浆凝血酶原时间、活化部分凝血活酶时间、凝血酶时间比较,差异均无统计学意义(P>0.05)。②VEGF、NGF、BDNF、NT-3和S100β存在广泛的PPI。PPI网络节点基因显著富集在Ras信号通路、神经营养因子信号通路和Rap1信号通路等KEGG通路上,显著富集在变性膜受体蛋白酪氨酸激酶信号通路、神经营养结合、轴突等GO功能项目上。结论低频超声联合纳米微泡可以促进老年脑血栓患者神经功能恢复,提高脑血栓老年患者神经营养因子水平,降低脑损伤程度。其潜在机制可能包括Ras信号通路、神经营养因子信号通路和Rap1信号通路等。Objective To investigate the effect of low-frequency ultrasound combined with nano-microbubbles therapy on neurological,motor function and serological marker levels in elderly patients with cerebral thrombosis,and to construct a protein-protein interaction(PPI)network based on serological markers.Methods①A total of 136 elderly patients with cerebral thrombosis treated in Xi an International Medical Center Hospital from March 2022 to September 2023 were selected.The patients were divided into observation group and control group by random number table,each had 68 cases.Both groups were treated with intravenous thrombolysis,i.e.Patients in observation group were treated with low-frequency ultrasound combined with nano-microbubbles therapy.Serum marker levels,coagulation function,nerve and motor function were compared between the two groups.②The PPI network was constructed using the String database.The gene ontology(GO)function enrichment analysis and the Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis were performed using R software.Results①After treatment,6 minute walking distanc,Barthel index score,upper limb Fugl-Meyer assessment(FMA)score and lower limb FMA score in observation group were higher than those in control group(P<0.05),and National Institute of Health Stroke Scale in observation group was lower than that in control group(P<0.05).After treatment,the serum levels of vascular endothelial growth factor(VEGF),S100 calcium binding protein B(S100β),nerve growth factor(NGF),Brain derived neurotrophic factor(BDNF)and neurotrophin 3(NT-3)in observation group were higher than those in control group(P<0.05).The serum S100βlevel in observation group was lower than that in control group(P<0.05).After treatment,there was no significant difference in prothrombin time,activated partial thromboplastin time,thrombin time between the two groups(P>0.05).②VEGF,NGF,BDNF,NT-3 and S100βhave extensive protein-protein interactions.PPI network node genes were significantly enriched in KEGG pathways s

关 键 词：脑血栓 低频超声 纳米微泡 

分 类 号：R74[医药卫生—神经病学与精神病学]