羊血红蛋白复合酶解物抑菌活性的研究  

Study on Antibacterial Activity of Hemoglobin Composite Enzymatic Hydrolysate of Sheep

作　　者：侯欣 訾阳 乔依娜 宝婧婧 冯雪莲 秦宇龙 刘迎春[2] 高峰[1] HOU Xin;ZI Yang;QIAO Yi’na;BAO Jingjing;FENG Xuelian;QIN Yulong;LIU Yingchun;GAO Feng(College of Animal Science,Inner Mongolia Agricultural University,Inner Mongolia Hohhot 010018,China;College of Life Sciences,Inner Mongolia Agricultural University,Inner Mongolia Hohhot 010018,China)

机构地区：[1]内蒙古农业大学动物科学学院,内蒙古呼和浩特010018 [2]内蒙古农业大学生命科学学院,内蒙古呼和浩特010018

出　　处：《饲料工业》2025年第5期110-115,共6页Feed Industry

基　　金：国家肉羊产业体系岗位科学家项目[CARS38];内蒙古自治区自然科学基金项目[2021MS03015];内蒙古自治区科技计划项目[2023YFHH0030];内蒙古自治区直属高校基本科研业务费项目[BR231402]。

摘　　要：试验旨在明确羊血红蛋白复合酶解物的抑菌活性。通过测定羊血红蛋白复合酶解物的抑菌圈直径、抑菌率、最小抑菌浓度(MIC)确定抑菌活性,分析细菌表面疏水性和培养液蛋白浓度,探究其抗菌机制。结果表明:羊血红蛋白复合酶解物对大肠埃希氏菌、肠沙门氏菌的抑菌圈直径分别为17.74、18.05 mm,对大肠埃希氏菌、肠沙门氏菌的最小抑菌浓度为20 mg/mL;在24 h时,60 mg/mL的羊血红蛋白复合酶解物对大肠埃希氏菌、肠沙门氏菌的抑菌率分别99.05%、87.55%;羊血红蛋白复合酶解物可使细菌疏水性增加,并使细胞内蛋白质泄漏。说明羊血红蛋白复合酶物对大肠埃希氏菌、肠沙门氏菌具有抑菌活性,且通过增加疏水性使细胞聚沉,破坏细胞膜完整性而达到抑菌效果。This paper aims to investigate the antibacterial activity of sheep hemoglobin complex hydroly⁃sate.The antibacterial activity was determined by measuring the diameter of the inhibition zone,inhibition rate,and minimum inhibitory concentration(MIC)of sheep hemoglobin complex enzymatic hydrolysate.The antibacterial mechanism was explored based on the hydrophobicity of the bacterial surface and the protein concentration in the culture medium.The inhibition zones′diameters of Escherichia coli and Salmonella enterica were measured as 17.74 mm and 18.05 mm for the sheep hemoglobin compound digest.The mini⁃mum inhibitory concentration of Escherichia coli and Salmonella enterica was found to be 20 mg/mL.At 24 hours,the inhibition rates of 60 mg/mL sheep hemoglobin complex digest against Escherichia coli and Salmonella enterica were recorded as 99.05%and 87.55%,respectively.Bacterial hydrophobicity was increased,and intracellular protein leakage was caused by the sheep hemoglobin complex digest.The results indicated that antibacterial activity against Escherichia coli and Salmonella enterica was exhibited by the sheep hemoglobin complex enzyme,which achieved its antibacterial effect by increasing hydrophobicity to aggregate cells and destroy the integrity of cell membranes.

关 键 词：羊血红蛋白 复合酶解物 抑菌圈直径 最小抑菌浓度 抑菌活性 

分 类 号：S816.7[农业科学—饲料科学]