Sheep-derived butyrate-producing Clostridium beijerinckii R8 alleviates diarrhea by shaping the gut microbiota of goat kids  

作　　者：Dingkun Fan Yuze Fu Jixian Zhang Yanliang Bi Tao Ma Qiyu Diao Naifeng Zhang 

机构地区：[1]Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs,Institute of Feed Research of Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《Animal Nutrition》2024年第4期13-24,共12页动物营养(英文版)

基　　金：funded by the Inner Mongolia Science and Technology Key Project(2021SZD0014,Inner Mongolia,China);the National Natural Science Foundation of China(31872385,Beijing,China).

摘　　要：Newborn goat kids exposed to environmental stress are susceptible to diarrhea due to immature intestinal functions and undeveloped gut microbiota.Butyrate-producing bacteria as next generation probiotics benefit the maintenance of intestinal health,but the mode of regulation is still unclear.Herein,a novel butyrate-producing strain was isolated from sheep rumen and identified as Clostridium beijerinckii(C.beijerinckii)R8,thereafter goat kids were treated with C.beijerinckii R8 to elucidate its regulatory mechanisms on diarrhea.Thirty-six goat kids were assigned to four groups:control(CON),low dose(LCB;supplementation with 5×10^(7)CFU/mL of C.beijerinckii R8,each with 10 mL/d),middle dose(MCB;supplementation with 5×10^(8)CFU/mL of C.beijerinckii R8,each with 10 mL/d),high dose(HCB;supplementation with 5×10^(9)CFU/mL of C.beijerinckii R8,each with 10 mL/d).The experiment lasted for 15 d,and 6 goat kids were randomly selected from each group for slaughter on the last day of the trial.The results showed that this isolate reduced the diarrhea rate(P<0.001)and fecal scores(P<0.001).In the gut,its supplementation inhibited inflammation,increased antioxidant capacity,and regulated intestinal flora.Meanwhile,C.beijerinckii R8 strengthened the intestinal barrier and altered the jejunum morphology.This evidence suggests that C.beijerinckii R8 may alleviate diarrhea in goat kids by regulating microbiota,which is directly related to intestinal barrier and immune capacity.

关 键 词：Clostridium beijerinckii DIARRHEA Intestinal microbiota Intestinal immune Intestinal barrier 

分 类 号：R57[医药卫生—消化系统]