Butyrate metabolism in rumen epithelium affected by host and diet regime through regulating microbiota in a goat model  

作　　者：Yimin Zhuang Mahmoud M.Abdelsattar Yuze Fu Naifeng Zhang Jianmin Chai 

机构地区：[1]Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding,College of Life Science and Engineering,Foshan University,Foshan 528225,China [2]Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs,Institute of Feed Research of Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China [4]Division of Agriculture,Department of Animal Science,University of Arkansas,Fayetteville,AR 72701,USA

出　　处：《Animal Nutrition》2024年第4期41-55,共15页动物营养(英文版)

基　　金：funded by grants from National Natural Science Foundation of China(31872385);Foshan Postdoctoral Sustentation Fund(BKS209151);the Inner Mongolia Science and Technology Key Project(2021SZD0014);the National Key R&D Program Projects(2018YFD0501902)。

摘　　要：The rumen is an important organ that enables ruminants to digest nutrients. However, the biological mechanism by which the microbiota and its derived fatty acids regulate rumen development is still unclear. In this study, 18 female Haimen goats were selected and slaughtered at d 30, 60, and 90 of age.Multi-omics analyses(rumen microbial sequencing, host transcriptome sequencing, and rumen epithelial metabolomics) were performed to investigate host-microbe interactions from preweaning to postweaning in a goat model. With increasing age, and after the introduction of solid feed, the increased abundances of Prevotella and Roseburia showed positive correlations with volatile fatty acid(VFA) levels and morphological parameters(P < 0.05). Epithelial transcriptomic analysis showed that the expression levels of hub genes, including 3-hydroxy-3-methylglutaryl-CoA synthase isoform 2(HMGCS2), enoyl-CoA hydratase, short chain 1(ECHS1), and peroxisome proliferator activated receptor gamma(PPARG), were positively associated with animal phenotype(P < 0.05). These hub genes were mainly correlated to VFA metabolism, oxidative phosphorylation, and the mammalian target of rapamycin(mTOR) and peroxisome proliferator activated receptor(PPAR) signaling pathways(P < 0.05). Moreover, the primary metabolites in the epithelium changed from glucose preweaning to(R)-3-hydroxybutyric acid(BHBA) and acetoacetic acid(ACAC) postweaning(P < 0.05). Diet and butyrate were the major factors shaping epithelial metabolomics in young ruminants(P < 0.05). Multi-omics analysis showed that the rumen microbiota and VFA were mainly associated with the epithelial transcriptome, and that alterations in gene expression influenced host metabolism. The “butanoate metabolism” pathway, which transcriptomic and metabolomic analyses identified as being upregulated with age, produces ketones that regulate the “oxidative phosphorylation” pathway, which could provide energy for the development of rumen papillae. Our findings reveal the changes that occur in th

关 键 词：Rumen microbiota Rumen epithelial development Multi-omics Host-microbe interaction 

分 类 号：R73[医药卫生—肿瘤]