Targeting gut microbiota and metabolism profiles with coated sodium butyrate to ameliorate high-energy and low-protein diet-induced intestinal barrier dysfunction in laying hens  

作　　者：Sasa Miao Jiankui Li Ying Chen Wenyan Zhao Mengru Xu Fang Liu Xiaoting Zou Xinyang Dong 

机构地区：[1]Key Laboratory of Animal Feed and Nutrition of Zhejiang Province,Key Laboratory of Animal Nutrition and Feed Science(Eastern of China),Ministry of Agriculture and Rural Affairs,The Key Laboratory of Molecular Animal Nutrition,Ministry of Education,College of Animal Sciences,Zhejiang University,Hangzhou 310058,China [2]Hangzhou Zhejiang University Animal Hospital Co.,Ltd.,Hangzhou 310058,China

出　　处：《Animal Nutrition》2024年第4期104-116,共13页动物营养(英文版)

基　　金：supported by the Twinning Service Plan of the Zhejiang Provincial Team Science and Technology Special Commissioner(Anji County,Huzhou,China),the Science and Technology Development Project of Hangzhou(202003A02);the Modern Argo-Industry Technology Research System of China(CARS-40-K10).

摘　　要：High energy diets are a risk factor for intestinal barrier damage.Butyrate,a major energy source for intestinal epithelial cells,has been shown to improve barrier dysfunction and modulate the gut microbiota.In this trial,we examined the preventative effect of coated sodium butyrate(CSB)on highenergy and low-protein(HELP)-induced intestinal barrier injury in laying hens,and also worked to determine the underlying mechanisms by an integrative analysis of gut microbiota and the metabolome.A total of 216 healthy 28-week-old Huafeng laying hens were randomly assigned to 3 groups with 6 replicates each:the CON group(normal diet),HELP group(HELP diet)and CH500 group(500 mg/kg CSB added to HELP diet).The duration of the trial encompassed a period of 10 weeks.The results revealed that CSB treatment improved the laying rate and mitigated the detrimental effects on intestinal barrier function and the inflammatory response induced by the HELP diet in laying hens(P<0.05).Microbial profiling analysis revealed that the CSB treatment reshaped the HELP-perturbed gut microbiota and promoted the growth of beneficial bacteria(P<0.05).Untargeted metabolomics analysis revealed that CSB reduced the metabolites associated with intestinal inflammation(P<0.05).In conclusion,CSB did not merely modulate alterations in the gut microbiota composition and microbial metabolites but also yielded increased egg production,while mitigating intestinal barrier dysfunction and inflammatory responses induced by HELP in laying hens.

关 键 词：Coated sodium butyrate Intestinal barrier High-energy and low-protein Gut microbiota Laying hen 

分 类 号：R57[医药卫生—消化系统]