Interactions between rumen epithelium-associated microbiota and host immunological and metabolic adaptations in response to different milk replacer feeding intensities in dairy calves  

作　　者：Carolin B.M.Müller Lisa-Maria Tümmler Henry Reyer Torsten Viergutz Björn Kuhla 

机构地区：[1]Institute for Farm Animal Biology(FBN),Dummerstorf 18196,Germany

出　　处：《Animal Nutrition》2024年第4期287-300,共14页动物营养(英文版)

基　　金：FACCE-JPI program and received funding from the German Federal Ministry of Food and Agriculture(BMEL)through the Federal Office for Agriculture and Food(BLE,Bonn,Germany),grant number 2814ERA04A;the epimural microbiome analysis was financially supported the FBN seed funding program.

摘　　要：The milk replacer feeding regime in dairy calves has a great impact on metabolic and immunological functioning and affects animal welfare and lifetime performance.The feeding regime influences the rumen microbial composition,and epithelium-associated microbes may interact with the immune system of the host.We examined the correlations between blood leukocyte counts and the rumen epithelium-associated microbiome in dairy calves fed 2 different milk replacer feeding intensities and if these factors related to metabolic traits.Fourteen newborn female dairy calves were allocated to a group receiving either 10%(n=7)or 20%(n=7)milk replacer of their body weight(on average 41 kg)and provided ad libitum access to grass hay and concentrate pellets.At 3 weeks of life,all calves were fitted with a rumen cannula.Calves were weaned at 12 weeks of life and received a total mixed ration for ad libitum intake.Pre-(8-10 weeks of life)and post-weaning(21-23 weeks of life),methane production was measured in respiration chambers,and rumen epithelium and blood were sampled for 16S rRNA sequencing and leukocyte analyses,respectively.Pre-weaning,the reduced milk replacer feeding intensity was accompanied with higher concentrate intake but lower growth performance(P<0.001),a higher abundance of amylolytic and lower abundance of cellulolytic epimural microbes.The group fed a low milk replacer intensity had also greater portions of monocytes(P=0.031),CD8^(+)(P<0.001),and CD14^(+)(P=0.044)leukocytes,suggesting elevated inflammatory conditions.Correlations between CD8^(+) T cells and rumen methanogens,Ruminococcaceae,and Lachnospiraceae were recorded,but these were not consistent throughout maturation.Post-weaning,differences in feed intake and rumen microbial composition converged among milk replacer groups,while differences in growth performance(P=0.040)and CD8^(+) cells(P<0.001)were still present.In conclusion,a reduced milk replacer feeding intensity in dairy calves compromised growth performance and immunity and this effect persisted i

关 键 词：Milk replacer Rumen microbe Immune system Host-microbe interaction 

分 类 号：R73[医药卫生—肿瘤]