Dietary astaxanthin alleviates black soldier fly oil-induced negative changes of fatty acids content and muscle quality on Oncorhynchus mykiss via mammalian target of rapamycin and AMP-activated protein kinase pathway  

作　　者：Yongkang Chen Jian Zhong Xuanqi Chen Xiaomin Li Haiqi Pu Baoyang Chen Yucai Guo Anqi Chen Wenjie Li Peng Hu Xinliang Zhu Wei Zhao Jin Niu 

机构地区：[1]State Key Laboratory of Biocontrol,Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),School of Life Sciences,Sun Yat-Sen University,Guangzhou 510275,China [2]Zhanjiang Customs District,Zhanjiang 524000,China [3]Qinghai Kunjie Environmental Protection Technology Co.,Ltd.,Xining 810000,China [4]Key Laboratory of Subcritical Extraction of Anyang City,Henan Subcritical Biotechnology Co.Ltd.,Anyang 455000,China

出　　处：《Animal Nutrition》2024年第4期313-324,共12页动物营养(英文版)

基　　金：supported by the Project of National Natural Science Foundation of China(32172982);National Natural Science Foundation of China(32303014);Guangdong Basic and Applied Basic Research Foundation(2024A1515010444);Project of Science and Technology of Guangdong Province(2023A1515012627).

摘　　要：This study evaluated the effect of black soldier fly(Hermetia illucens)larvae oil(BO)produced by a novel technique,subcritical butane extraction,on the flesh quality,lipid nutrients and muscle growth of rainbow trout(Oncorhynchus mykiss)fillet,and investigated the alleviating mechanisms of dietary astaxanthin(AST)supplementation.Two hundred and forty fish(215.16±2.30 g)were distributed to three groups with four replicates.Fish were fed three experimental diets for 8 weeks:the control diet(CD diet),total fish oil of the CD diet was replaced with BO to formulate the BO100 diet,and then 1 g/kg AST was supplemented with the BO100 diet to formulate the AST diet.Results showed that the final body weight and the sarcomere length of fillet were significantly increased and the protein phosphorylation levels of mammalian target of rapamycin(mTOR)and p70 S6 kinase were enhanced in the BO100 group compared to the CD group(P<0.05).However,there was a reduction in the hardness,springiness and chewiness of fillets,with a decrease in eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)levels in the fish of the BO100 group(P<0.05).Supplementation of AST in the BO100 diet mitigated the impairment of springiness and chewiness and further promoted the sarcomere length of fillet(P<0.05).Furthermore,dietary AST partially restored the EPA and DHA content of fillet by increasing the phosphorylation levels of serine/threonine kinase(AKT)and AMP-activated protein kinase α(AMPKα)(P<0.05)and activating the gene expression of unsaturated fatty acid synthesis.To conclude,BO produced by subcritical butane extraction can be a readily available oil source for rainbow trout feed that can be used to promote muscle growth in rainbow trout.Further dietary AST supplementation can alleviate BO-induced lipid accumulation,restore DHA levels and improve the flesh quality of rainbow trout fillet.

关 键 词：Black soldier fly larvae oil ASTAXANTHIN Flesh quality DHA mTOR pathway 

分 类 号：R73[医药卫生—肿瘤]