Dietary high lipid and high plant-protein affected growth performance,liver health,bile acid metabolism and gut microbiota in groupers  

作　　者：Jia Xu Fan Wang Chaoqun Hu Junxiang Lai Shiwei Xie Kefu Yu Fajun Jiang 

机构地区：[1]Guangxi Key Laboratory of Marine Environmental Science,Guangxi Academy of Marine Sciences,Guangxi Academy of Sciences,Nanning 530007,China [2]Guangxi Laboratory on the Study of Coral Reefs in the South China Sea,School of Marine Sciences,Guangxi University,Nanning 530004,China [3]Key Laboratory of Tropical Marine Bio-Resources and Ecology,Guangdong Provincial Key Laboratory of Applied Marine Biology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China [4]Laboratory of Aquatic Animal Nutrition and Feed,Fisheries College,Guangdong Ocean University,Zhanjiang 524088,China

出　　处：《Animal Nutrition》2024年第4期370-385,共16页动物营养(英文版)

基　　金：supported by the fund of the Natural Science Foundation of Guangxi(2023GXNSFBA026052);Guangxi Academy of Sciences Basic Research Operating Expenses for Innovative Team Initiation Grant Project(CQ-C-202301);Key Research and Development Program of Guangxi(AB23075180);Guangxi mangrove coastal wetland ecological protection and sustainable use of small highland talents project(BGMRC202104).

摘　　要：High lipid diets(HLD)and high plant-protein diets(HPD)exhibit potential fishmeal-saving effects but negatively impact liver health and growth performance in fish.We hypothesized that HLD and HPD impair liver health in pearl gentian groupers(Epinephelus fuscoguttatus♂×Epinephelus lanceolatus♀)through the modulation of intestinal microbiota and bile acid(BA)metabolism.Four diet groups were tested:control diet(CD,46.21%crude protein,9.48%crude lipid),HLD(46.37%crude protein,16.70%crude lipid),HPD(46.50%crude protein,9.38%crude lipid),and high lipid-high plant-protein diet(HLPD,46.54%crude protein,16.67%crude lipid).A total of 300 fish(average body weight=15.22±0.03 g)were randomly divided into 4 diet treatments(ensuring 3 tanks replicates of each diet treatment,each tank containing 25 fish).After an eight-week feeding period,the HLD and HPD significantly decreased the final body weight(FBW),weight gain rate(WGR),specific growth rate(SGR)and feed intake(FI)in comparison to CD group,with HLPD exacerbating these indicators(P<0.05).Compared to CD group,the content of total cholesterol(T-CHO)and triglyceride(TG)in liver and serum were significantly increased in HLD group(P<0.05).Compared to HPD group,the content of T-CHO in liver was significantly decreased,the content of TG in liver and serum were significantly increased in HLPD group(P<0.05).HLD,HPD,and HLPD impaired liver health by inducing histological damage,inflammation,and oxidative stress.Compared to CD group,the mRNA relative expression of bile salt export pump(bsep)and multidrug resistance protein 3(mdr3)were significantly increased in HLD group,whereas the mRNA relative expression of sterol-27-hydroxylase(cyp27a1),microsomal epoxide hydrolase(meh),apical sodium-dependent bile acid transporter(asbt),multidrug resistance-associated protein 3(mrp3),farnesoid X receptor(fxr)and G protein-coupled bile acid receptor 5(tgr5)were significantly decreased(P<0.05).Compared to CD group,the mRNA relative expression of mdr3,asbt,mrp3,organic anion transporters 1(oatp1

关 键 词：Liver damage Lipid metabolism Bile acid metabolism MICROBIOTA GROUPER 

分 类 号：R74[医药卫生—神经病学与精神病学]