河南省农村饮用水环境抗生素抗性基因的赋存特征及影响因素分析  

Occurrence Characteristics and Influencing Factors of Antibiotic Resistance Genes in Rural Drinking Water in Henan Province

作　　者：张凯 马鸽 李贶家 邹威 杨凤霞 辛蕊 黄耀 邱燕子 史超格 王倩 ZHANG Kai;MA Ge;LI Kuang-jia;ZOU Wei;YANG Feng-xia;XIN Rui;HUANG Yao;QIU Yan-zi;SHI Chao-ge;WANG Qian(Henan Key Laboratory for Synergistic Prevention of Water and Soil Environmental Pollution,School of Geographic Sciences,Xinyang Normal University,Xinyang 464000,China;Development Research Center,Ministry of Water Resources,Beijing 100038,China;Henan Key Laboratory of Environmental Pollution Control,Key Laboratory for Yellow River and Huai River Water Environment and Pollution Control,Ministry of Education,College of Environment,Henan Normal University,Xinxiang 453007,China;Agro-Environmental Protection Institute,Ministry of Agriculture and Rural,Tianjin 300191,China;Institute of Eco-environmental and Soil Sciences,Guangdong Academy of Sciences,Guangzhou 510650,China)

机构地区：[1]信阳师范大学地理科学学院,河南省水土污染协同防治重点实验室,信阳464000 [2]水利部发展研究中心,北京100038 [3]河南师范大学环境学院,黄淮水环境污染与防治教育部重点实验室,河南省环境污染控制重点实验室,新乡453007 [4]农业农村部环境保护科研监测所,天津300191 [5]广东省科学院生态环境与土壤研究所,广州510650

出　　处：《环境科学》2025年第3期1427-1434,共8页Environmental Science

基　　金：国家自然科学基金项目(42377280);河南省优秀青年科学基金项目(232300421102);云南省基础研究计划项目(202401AT070304);信阳师范学院“南湖学者奖励计划”青年项目。

摘　　要：抗生素抗性基因(ARGs)作为一种新污染物能够使用于临床治疗的抗生素失效,环境是其重要的储存库,因而ARGs在环境中的迁移转化近年来备受关注.然而,目前农村饮用水环境中ARGs赋存状况的研究仍相对匮乏.以农业大省河南为研究区域,采集了35个农村饮用水样品,对7类共16种ARGs以及整合子intI1进行了定量.结果表明,磺胺类ARGs是河南省农村饮用水环境中分布最为广泛且污染最为严重的耐药基因型.四环素类ARGs丰度虽然仅次于磺胺类ARGs,但其检出率较低.氯霉素类ARGs的平均相对丰度及检出率均处于较低水平,初步表明河南农村饮用水环境中非主要使用抗生素的ARGs造成的污染较轻. intI1与多数ARGs均有一定的正相关关系,表明其对ARGs在饮用水环境中的传播扩散起到了关键作用. GDP则对ARGs的分布没有影响,表明各地对农村环境中ARGs污染防控投入差别较小.不同水源地类型的饮用水ARGs丰度差别较小,饮用水处理工艺对源水中ARGs的去除效果可能是造成这一现象的重要原因.Antibiotic resistance genes(ARGs)as a novel type of pollutant can lead to the ineffectiveness of antibiotics used in clinical treatment.The environment serves as an important reservoir for ARGs,and therefore,the migration and transformation of ARGs in the environment have attracted increasing attention in recent years.However,studies on the presence of ARGs in rural drinking water environments remain scarce.This study focused on Henan Province,a major agricultural province,where 35 samples of rural drinking water were collected and quantitatively analyzed for 16 types of ARGs belonging to seven classes,as well as the integrase gene intI1.The results showed that sulfonamide ARGs were the most widely distributed and heavily contaminated ARG types in rural drinking water environments in Henan Province.Although tetracycline ARGs ranked second to sulfonamide ARGs in terms of content,their detection rate was lower.Chloramphenicol ARGs had relatively low average relative abundance and detection rates,indicating that contamination from ARGs of antibiotics less commonly used in the rural drinking water environment in Henan was less severe.intI1 showed a positive correlation with most ARGs,suggesting its crucial role in the dissemination of ARGs in the drinking water environment.GDP did not affect the distribution of ARGs,indicating less difference in investment in the prevention and control of ARGs pollution in rural environments across different regions.The abundance of ARGs in drinking water from different types of water sources showed negligible difference,and the removal efficiency of ARGs from source water by drinking water treatment processes may be an important factor contributing to this phenomenon.

关 键 词：河南省 农村 饮用水 抗生素抗性基因(ARGs) 赋存特征 

分 类 号：X131.2[环境科学与工程—环境科学]