双波长紫外协同强化消毒及对光、暗复活影响  

Synergistic Disinfection Effects of Dual-wavelength Ultraviolet and Its Impact on Photoreactivation and Dark Repair

作　　者：周添红[1] 梁源夫 孙喆 李梦凯[2] 王佳乐 李文涛[2] 贲伟伟[2] 张国珍[1] 强志民[2] ZHOU Tian-hong;LIANG Yuan-fu;SUN Zhe;LI Meng-kai;WANG Jia-le;LI Wen-tao;BEN Wei-wei;ZHANG Guo-zhen;QIANG Zhi-min(School of Environment and Municipal Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China;State Key Laboratory of Environmental Aquatic Chemistry,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China)

机构地区：[1]兰州交通大学环境与市政工程学院,兰州730070 [2]中国科学院生态环境研究中心环境水质学国家重点实验室,北京100085

出　　处：《环境科学》2025年第3期1443-1449,共7页Environmental Science

基　　金：中国科学院国际合作局国际伙伴计划项目(032GJHZ2022035MI);国家自然科学基金项目(52222002,52091545);甘肃省教育厅高校科研创新平台重大培育项目(2024CXPT-14)。

摘　　要：C波段紫外线(UV-C)可有效灭活水中病原微生物,远UV-C(200~230 nm)和常规UV-C(250~280 nm)可分别损伤病原微生物的蛋白质和核酸,同时辐照有望实现病原微生物的协同灭活.然而由于现有实验装置难以满足研究需求,相关研究十分有限.因此,搭建了配装KrCl准分子灯和低压汞灯的细管流光反应系统,可单独/同时输出稳定的222 nm(远UV-C)和254 nm(常规UVC)辐照.之后利用该装置探究了双波长UV-C对大肠杆菌(E.coli)的协同灭活作用,以及对光、暗复活的影响.结果表明,双波长UV-C协同灭活E.coli作用显著,协同系数最高可达2.2.此外,双波长UV-C辐照明显减弱E.coli的光复活作用,最大对数复活率从254 nm单独辐照的50.8%降低为36.1%,同时,在黑暗条件下,未发现复活现象且呈现暗凋零现象,说明双波长UV-C辐照可降低光、暗复活风险,确保水质生物安全,研究可为高效和安全的水消毒技术研发提供理论依据.Ultraviolet-C(UV-C)irradiation can effectively deactivate pathogenic microorganisms.The far UV-C(200-230 nm)and conventional UV-C(250-280 nm)can damage the proteins and nucleic acids of pathogenic microorganisms,respectively.The combination of far and conventional UV-C has the potential of synergistic inactivation.However,relevant studies remain limited owing to the lack of appropriate experimental setups.Therefore,this study established a mini-fluidic photoreaction system equipped with a KrCl excimer lamp and a low-pressure mercury lamp.This system could independently/simultaneously deliver stable 222 nm(far UV-C)and 254 nm(conventional UV-C)irradiations.Subsequently,the system was used to investigate the synergistic effect of dual-wavelength UV-C(the combination of 222 nm and 254 nm UV-C)on Escherichia coli(E.coli)inactivation and the subsequent impact on photo-and dark-reactivation.The results indicated that dual-wavelength UV-C had a significant synergistic effect on E.coli inactivation,with a synergistic coefficient up to 2.2.Additionally,comparing to 254 nm UV irradiation,the photo-reactivation of E.coli after dual-wavelength UV-C irradiation was weakened,with the maximum lg reactivation percentage reducing from 50.8%to 36.1%.Furthermore,E.coli after dual-wavelength UV-C irradiation exhibited dark decay,in which the inactivation efficiency was further enhanced during dark treatment.Therefore,these results suggest that dual-wavelength UV-C could inhibit the light reactivation and dark repair of E.coli,ensuring the water biosafety,as well as providing reference for the development of effective and safe water disinfection technologies.

关 键 词：紫外线 消毒 双波长 协同作用 微生物复活 

分 类 号：X131.2[环境科学与工程—环境科学]