土壤Cd胁迫条件下外源NO与EDDS复合处理对三叶鬼针草生长及Cd积累的影响  

Effects of Exogenous NO Combined with EDDS on Growth and Cd Accumulation of Bidens pilosa under Soil Cd Stress Conditions

作　　者：卢誉之 陈银萍[1] 颉海帆 曹渤 李倩 孙勇 袁巧玲 LU Yu-zhi;CHEN Yin-ping;XIE Hai-fan;CAO Bo;LI Qian;SUN Yong;YUAN Qiao-ling(Key Laboratory of Strategic Mineral Resources of the Upper Yellow River,Ministry of Natural Resources,College of Environmental and Municipal Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China)

机构地区：[1]兰州交通大学环境与市政工程学院,自然资源部黄河上游战略性矿产资源重点实验室,兰州730070

出　　处：《环境科学》2025年第3期1783-1794,共12页Environmental Science

基　　金：国家自然科学基金项目(32260316);自然资源部黄河上游战略性矿产资源重点实验室2023年度开放基金项目(YSMRKF202306)。

摘　　要：在40 mg·kg^(-1)Cd胁迫条件下,采用盆栽法探究了0.05、0.10、0.20和0.30 mmol·L^(-1)硝普钠(SNP)及0.50、1.00和1.50 mmol·L^(-1)[S,S]-乙二胺二琥珀酸(EDDS)单一和复合处理对三叶鬼针草(Bidens pilosa)幼苗生长代谢和Cd积累的影响.结果表明,与对照组(不添加SNP和EDDS)相比,不同浓度SNP或EDDS单一处理能显著提高三叶鬼针草的株高、根长、地上部和地下部的鲜干重,并有效增加光合色素含量以及地上部和地下部Cd含量;不同浓度SNP和EDDS复合处理能有效促进三叶鬼针草生长,部分生长指标和Cd积累指标高于同浓度SNP和EDDS的单一处理,其中,0.20 mmol·L^(-1)SNP+1.00 mmol·L^(-1)EDDS复合处理:①显著促进三叶鬼针草的生物量,促使株高、地上部和地下部的鲜干重分别增大了39.47%、142.32%和267.63%、377.63%和304.55%;②显著提高三叶鬼针草对Cd污染土壤的修复效果,分别促使地上部和地下部Cd含量、地上部和地下部富集系数、修复效率和转运系数增加了91.22%和81.51%、101.71%和91.37%、641.67%和5.14%,且地上部的Cd含量和富集系数大幅度高于地下部;③显著提高三叶鬼针草亚细胞各组分的Cd含量,其中细胞壁和可溶部分的Cd含量高于细胞器和线粒体部分,地上部亚细胞组分Cd含量也均大于地下部.综合分析表明:在土壤Cd胁迫下,施用适宜浓度的SNP和EDDS能有效促进三叶鬼针草生长和Cd富集,有助于三叶鬼针草通过细胞壁的屏障作用和液泡的区室化作用增强Cd抗性和解毒作用.根据试验结果认为,可选用0.20 mmol·L^(-1)SNP+1.00 mmol·L^(-1)EDDS复合处理增强三叶鬼针草修复Cd污染土壤的效率.Under 40 mg·kg^(−1) Cd stress,pot experiments were conducted to investigate the effects of 0.05,0.10,0.20,and 0.30 mmol·L^(−1) sodium nitroprusside(SNP)and 0.50,1.00,and 1.50 mmol·L^(−1)[S,S]-ethylenediamine disuccinic acid(EDDS)on the growth,metabolism,and Cd accumulation of Bidens pilosa seedlings.The results showed that compared with those of the control group(without SNP and EDDS),different concentrations of SNP or EDDS single treatment could significantly increase the plant height,root length,fresh weight,and dry weight of aboveground and underground parts of B.pilosa and effectively increase the photosynthetic pigment content and Cd content in the aboveground and underground parts.The combined treatment of different concentrations of SNP and EDDS could effectively promote the growth of B.pilosa,and some growth indices and Cd accumulation indices were higher than those of the single treatment of SNP and EDDS at the same concentration.Among them,the 0.20 mmol·L^(−1) SNP+1.00 mmol·L^(−1) EDDS composite treatment had the following effects:①The treatment significantly promoted the biomass of B.pilosa and increased the plant height and fresh and dry weight of aboveground and underground parts by 39.47%,142.32%and 267.63%,and 377.63%and 304.55%,respectively.②It significantly improved the remediation effect of B.pilosa on Cdcontaminated soil,and the Cd content,enrichment coefficient,remediation efficiency,and transport coefficient of aboveground and underground parts of B.pilosa were increased by 91.22%and 81.51%,101.71%,and 91.37%,641.67%,and 5.14%,respectively,and the Cd content and enrichment coefficient of aboveground parts were significantly higher than those of underground parts.③The treatment also significantly increased the Cd content in subcellular fractions of B.pilosa,the Cd content in the cell wall and soluble part was higher than that in the organelle and mitochondrial parts,and the Cd content in subcellular fractions of the aboveground part was also higher than that of the undergr

关 键 词：外源NO [S S]-乙二胺二琥珀酸(EDDS) 土壤CD污染 三叶鬼针草 生长 Cd积累 

分 类 号：X171.5[环境科学与工程—环境科学]