非肌层浸润性膀胱癌患者术前血清miR-29表达及其临床意义  

Preoperative Serum miR-29 Expression in Patients with Non Muscle Invasive Bladder Cancer and its Clinical Significance

作　　者：王茜 石梦侠 崔媛媛 刘相臣 WANG Qian;SHI Mengxia;CUI Yuanyuan(Kaifeng Central Hospital,Kaifeng,475000)

机构地区：[1]河南省开封市中心医院,475000

出　　处：《实用癌症杂志》2025年第3期380-383,共4页The Practical Journal of Cancer

摘　　要：目的 探讨非肌层浸润性膀胱癌(NMIBC)患者术前血清m iR-29表达及其临床意义。方法 纳入160例行经尿道膀胱肿瘤电切术(TURBT)治疗的NMIBC患者,设为观察组;纳入同期体检的150例健康体检者,设为对照组。采集所有研究对象5 ml空腹静脉血,采用实时荧光定量PCR法检测血清miR-29水平,比较两组血清miR-29水平表达差异。观察组术后随访2年,分析术后复发的影响因素,并绘制受试者工作特征(ROC)曲线,探讨术前血清miR-29对NMIBC术后复发的预测价值。结果 观察组血清miR-29表达量低于对照组(P<0.05)。TNM分期Ⅲ期、低分化患者术前血清miR-29水平低于TNM分期Ⅰ~Ⅱ期、中高分化患者(P<0.05)。共158例患者完成随访。158例NMIBC患者TURBT术后复发32例,复发率为20.25%。复发组术前血清miR-29表达量低于未复发组(P<0.05)。复发组TNM分期Ⅲ期、低分化占比高于未复发组(P<0.05)。经Logistic回归分析检验显示,术前血清miR-29过表达是术后复发的保护因素(OR<1,P<0.05)。绘制ROC曲线,术前血清miR-29预测NMIBC患者TURBT术后复发AUC值为0.801,预测价值中等。结论 NMIBC患者术前miR-29水平呈低表达,与肿瘤临床分期、分化程度联系紧密;可能与TURBT术后复发有关,开展NMIBC患者术前miR-29检查有助于预测TURBT术后复发。Objective To investigate the expression and clinical significance of miR-29 in preoperative serum of patients with non muscle invasive bladder cancer(NMIBC).Methods A total of 160 patients with NMIBC who underwent transurethral resection of bladder tumor(TURBT)were included as the observation group.150 healthy individuals who underwent physical examinations during the same period were included in the control group.5ml fasting venous blood of all subjects was collected,and serum miR-29 level was detected by real-time fluorescence quantitative PCR.The difference of serum miR-29 level between the 2 groups was compared.The observation group was followed up for 2 years after surgery to analyze the influencing factors of postoperative recurrence and draw receiver operating characteristic(ROC)curves to explore the predictive value of preoperative serum miR-29 for postoperative recurrence of NMIBC.Results The serum miR-29 expression level in the observation group was lower than that in the control group(P<0.05).The preoperative serum miR-29 levels in TNM stageⅢand poorly differentiated patients were lower than those in TNM stageⅠ~Ⅱand well differentiated patients(P<0.05).A total of 158 patients completed follow-up.32 out of 158 NMIBC patients experienced postoperative recurrence after TURBT,with a recurrence rate of 20.25%.The preoperative serum miR-29 expression level in the recurrent group was lower than that in the non recurrent group(P<0.05).The proportion of TNM stageⅢand low differentiation in the recurrent group was higher than that in the non recurrent group(P<0.05).Logistic regression analysis showed that overexpression of miR-29 in preoperative serum was a protective factor for postoperative recurrence(OR<1,P<0.05).The ROC curve was drawn.The AUC value of preoperative serum miR-29 in predicting recurrence after TURBT in NMIBC patients was 0.801,and the predictive value was moderate.Conclusion Preoperative miR-29 levels are low in NMIBC patients,closely related to tumor clinical staging and differentiati

关 键 词：非肌层浸润性膀胱癌 微小RNA-29 术后复发 预测价值 

分 类 号：R737.14[医药卫生—肿瘤]