Single-nucleus transcriptomics and chromatin accessibility analysis of musk gland development in Chinese forest musk deer(Moschus berezovskii)  

作　　者：Chenmiao LIU Tingting HONG Chengcheng ZHAO Tao XUE Shuhui WANG Zhanjun REN 

机构地区：[1]College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,China

出　　处：《Integrative Zoology》2024年第5期955-974,共20页整合动物学(英文版)

基　　金：supported by the Science and Technology Project of the State Forestry Administration(20180109000005);the Science and Technology Promotion Project of Shaanxi Forestry Bureau(20180611000001);the Science and Technology Project of the National Forestry and Grass Administration(20191202000009);the Shaanxi Academy of Forestry Sciences(SXL(2020-0207)).

摘　　要：Musk secreted by male forest musk deer(Moschus berezovskii)musk glands is an invaluable component of medicine and perfume.Musk secretion depends on musk gland maturation;however,the mechanism of its development remains elusive.Herein,using single cell multiome ATAC+gene expression coupled with several bioinformatic analyses,a dynamic transcriptional cell atlas of musk gland development was revealed,and key genes and transcription factors affecting its development were determined.Twelve cell types,including two different types of acinar cells(Clusters 0 and 10)were identified.Single-nucleus RNA and single-nucleus ATAC sequencing analyses revealed that seven core target genes associated with musk secretion(Hsd17b2,Acacb,Lss,Vapa,Aldh16a1,Aldh7a1,and Sqle)were regulated by 12 core transcription factors(FOXO1,CUX2,RORA,RUNX1,KLF6,MGA,NFIC,FOXO3,ETV5,NR3C1,HSF4,and MITF)during the development of Cluster 0 acinar cells.Kyoto Encyclopedia of Genes and Genomes enrichment showed significant changes in the pathways associated with musk secretion during acinar cell development.Gene set variation analysis also revealed that certain pathways associated with musk secretion were enriched in 6-year-old acinar cells.A gene co-expression network was constructed during acinar cell development to provide a precise understanding of the connections between transcription factors,genes,and pathways.Finally,intercellular communication analysis showed that intercellular communication is involved in musk gland development.This study provides crucial insights into the changes and key factors underlying musk gland development,which serve as valuable resources for studying musk secretion mechanisms and promoting the protection of this endangered species.

关 键 词：acinar cells Chinese forest musk deer musk gland development single cell multiome ATAC+gene expression 

分 类 号：R73[医药卫生—肿瘤]