9种非特异性免疫因子在2个年龄组大鲵中的组织分布及活性比较  

Comparison of tissue distribution and activity of nine non-specific immune factors in two age groups of Chinese giant salamander(Andrias davidianus)

作　　者：钱璟 梅亚洁 郭琳 吴思念 史明艳[1] 陈万光[1] QIAN Jing;Mei Yajie;Guo Lin;Wu Sinian;SHI Mingyan;Chen Wanguang(Luoyang Normal University,Research Center of Aquaculture Engineering Technology of Cold Water Fish in Henan Province,Scientific Observation and Research Station for Yellow River Wetland Ecosystem in Western Henan Region,Luoyang,Henan 471934,China;Yichuan Siyuan Experimental School,Luoyang,Henan 471399,China)

机构地区：[1]洛阳师范学院,河南省冷水鱼养殖工程技术研究中心,河南省豫西地区黄河湿地生态系统野外科学观测研究站,河南洛阳471934 [2]伊川思源实验学校,河南洛阳471399

出　　处：《水产科技情报》2025年第2期108-115,共8页Fisheries Science & Technology Information

摘　　要：为探究大鲵非特异性免疫因子的组织分布和活性变化,利用化学比色法和酶联免疫吸附法对2个年龄组(1龄和2龄)大鲵肝脏、肠道和脾脏中的一氧化氮(NO)、总一氧化氮合酶(TNOS)、诱导型一氧化氮合酶(iNOS)、溶菌酶(LYZ)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、补体C3(C3)、补体C4(C4)和转铁蛋白(Tf)等9种非特异性免疫因子进行了测定。结果显示:9种非特异性免疫因子在2个年龄组大鲵的3个组织中均有分布,但其活性在不同年龄组和不同组织中存在一定的差异。1龄大鲵脾脏中LYZ、ACP、AKP和Tf的活性高于肝脏和肠道,肝脏中NO、C3和C4的活性高于脾脏和肠道,肠道中TNOS和iNOS的活性高于脾脏和肝脏;2龄大鲵肝脏中NO、TNOS、iNOS、C3和C4的活性高于脾脏和肠道,肠道中LYZ和AKP的活性高于脾脏和肝脏,脾脏中ACP和Tf的活性高于肝脏和肠道。结果表明,9种非特异性免疫因子在1龄大鲵中主要分布于脾脏和肝脏,而2龄大鲵主要分布于肝脏。To investigate the tissue distribution and activity changes of non-specific immune factors in Chinese giant salamander(Andrias davidianus),the contents of nitric oxide(NO),total nitric oxide synthase(TNOS),inducible nitric oxide synthase(iNOS),lysozyme(LYZ),acid phosphatase(ACP),alkaline phosphatase(AKP),complement C3(C3),complement C4(C4)and transferrin(Tf)were determined in the liver,intestine,and spleen of two age groups of A.davidianus using chemical colorimetric methods and enzyme-linked immunosorbent assay(ELISA).The results showed that the nine non-specific immune factors were distributed in all these three tissues,while their activities varied between different age groups and tissues.In the one-year-old A.davidianus,the activities of LYZ,ACP,AKP and Tf in the spleen were higher than those in the liver and intestine.The activities of NO,C3 and C4 in the liver were higher than those in the spleen and intestine,while the activities of TNOS and iNOS in the intestine were higher than those in the spleen and liver.In the two-year-old A.davidianus,the activities of NO,TNOS,iNOS,C3 and C4 in the liver were higher than those in the spleen and intestine.The activities of LYZ and AKP in the intestine were higher than those in the liver and spleen,while the activities of ACP and Tf in the spleen were higher than those in the liver and intestine.The results of this study indicated that the spleen and the liver were the main distribution areas of these non-specific immune factors in the one-year-old A.davidianus,while the liver was the main distribution area of these non-specific immune factors in two-year-old A.davidianus.

关 键 词：大鲵 非特异性免疫因子 免疫组织 组织分布 活性变化 

分 类 号：R73[医药卫生—肿瘤]