钛等离子体浸没离子注入增强Bio-HPP材料的细胞相容性和抗菌性  

Titanium plasma immersion ion implantation enhanced cytocompatibility and antibacterial activity of Bio-HPP materials

作　　者：许朗 黄忠卫 石锐 XU Lang;HUANG Zhong-wei;SHI Rui(Department of Stomatology,Beijing Jishuitan Hospital,Capital Medical University,Beijing 100035,China;Department of Stomatology,Beijing Royal Integrative Traditional Medicine Hospital,Beijing 102200,China;Beijing Research Institute of Traumatology and Orthopaedics/Research Institute of Beijing Jishuitan Hospital Affiliated to Capital Medical University,Beijing 100035,China)

机构地区：[1]首都医科大学附属北京积水潭医院口腔科,北京100035 [2]北京王府中西医结合医院口腔科,北京102200 [3]北京市创伤骨科研究所/首都医科大学附属北京积水潭医院研究所,北京100035

出　　处：《局解手术学杂志》2025年第3期187-192,共6页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金面上项目(82072406)。

摘　　要：目的使用等离子体浸没离子注入(PIII)技术将钛离子注入到Bio-HPP材料表面,并初步评价该材料对人牙龈成纤维细胞(HGFs)的生物相容性和对牙龈卟啉单胞菌(P.g)的抗菌性。方法体外研究为细胞实验和抗菌实验,将试件随机分为改性组和对照组,改性组试件置于离子注入装置内,采用PIII技术注入钛离子。通过扫描电子显微镜观察材料表面纳米结构的形态。通过HGFs的增殖检测、免疫荧光染色和细胞周期分布检测进行Bio-HPP材料细胞相容性评估。进行P.g的抗菌率检测和致病基因表达分析来评价材料的抗菌活性,初步分析其抗菌机制。结果对照组Bio-HPP表面相对平整,改性组形成了直径为100~300 nm的纳米孔,纳米结构中分布着更小的纳米颗粒。培养3、5、7 d后,改性组细胞增殖率均高于对照组(P<0.05)。孵育4 h后,细胞在2组材料表面均有较好的铺展,细胞骨架进一步向外延伸,但对照组样品表面的铺展面积略小于改性组。孵育24 h后,2组材料表面细胞均呈细长的纺锤形或梭形,生长良好。对照组和改性组G1和G2期细胞比例比较,差异无统计学意义(P>0.05);改性组S期细胞比例高于对照组(P<0.05)。培养24 h后,改性组菌落数相对于对照组显著减少;改性组的抗菌率明显高于对照组(P<0.05)。改性组表面致病基因fimA相对表达量明显低于对照组(P<0.05)。结论钛等离子体注入改性的Bio-HPP材料促进了HGFs的黏附和增殖,有利于牙龈周围软组织黏附,且具有较好的抗菌活性。Objective Titanium ions were implanted into Bio-HPP materials using plasma immersion ion implantation(PIII)technology,and the biocompatibility against human gingival fibroblasts(HGFs)and antimicrobial activity against porphyromonas gingivalis(P.g)to the materials were preliminarily evaluated.Methods The cell experiments and antibacterial experiments in vitro were conducted,and the specimens were randomly divided into the modified group and the control group,and the modified specimens were placed in an ion implantation device and implanted with titanium ions by PIII technology.The morphology of the nanostructures on the surface of the materials was observed by scanning electron microscopy.The cytocompatibility of Bio-HPP materials was evaluated by proliferation detection,immunofluorescence staining and cell cycle distribution detection of HGFs.The antibacterial activity of the materials was evaluated by the detection of antibacterial rate of P.g and the expression analysis of pathogenic gene,and the antibacterial mechanism was preliminarily analyzed.Results The surface of Bio-HPP in the control group was relatively flat,and the modified group formed nanopores with diameters of 100 to 300 nm,with smaller nanoparticles distributed in the nanostructures.The cell proliferation rates of HGFs after cultured for 3,5 and 7 days in the modified group were higher than those in the control group(P<0.05).After 4 hours of incubation,the cells spread well on the surface of the both groups,and the cytoskeleton extended further outward,while the spreading area on the surface of the control group was slightly smaller than that of the modified group.After 24 hours of incubation,the cells on the surface of materials in the both groups showed elongated spindle or shuttle shape,with good growth.There was no significant difference in the proportions of cells at stages G1 and G2 between the control group and the modified group(P>0.05).The proportion of cells at stage S in the modified group was higher than that in the control group(P<0.

关 键 词：人牙龈成纤维细胞 Bio-HPP 黏附 增殖 牙龈卟啉单胞菌 抗菌性 

分 类 号：R783.1[医药卫生—口腔医学] R318.08[医药卫生—临床医学]