外源类黄酮对尾巨桉-降香黄檀间作体系幼苗生长和生理的影响  

Effects of Exogenous Flavonoids on Seedlings Growth and Physiology of Eucalyptus urophylla×E.grandis-Dalbergia odorifera in Intercropping System

作　　者：李林欣 韦庆琳 邓家珍 黄永珍 张涵 叶绍明[1,4,5] LI Linxin;WEI Qinglin;DENG Jiazhen;HUANG Yongzhen;ZHANG Han;YE Shaoming(College of Forestry,Guangxi University,Nanning 530004,Guangxi,China;Guangxi Zhuang Autonomous RegionState-owned GaofengForest Farm,Nanning 537599,Guangxi,China;Guangxi Zhuang Autonomous RegionState-owned DongmenForest Farm,Chongzuo 532108,Guangxi,China;Guangxi Colleges and University Key Laboratory for Cultivation and Utilization of Subtropical Forest Plantation,Nanning 530004,Guangxi,China;Guangxi Key Laboratory of Forest Ecology and Conservation,Nanning 530004,Guangxi,China)

机构地区：[1]广西大学林学院,广西南宁530004 [2]广西壮族自治区国有高峰林场,广西南宁537599 [3]广西壮族自治区国有东门林场,广西崇左532108 [4]广西高校亚热带人工林培育与利用重点实验室,广西南宁530004 [5]广西森林生态与保育重点实验室,广西南宁530004

出　　处：《桉树科技》2025年第1期1-15,共15页Eucalypt Science & Technology

基　　金：国家自然科学基金项目(32260382);国家自然科学基金项目(31460169);中央财政林业科技推广项目(2022TG26号);广西自筹经费林业科技项目(2023GXZCLK09)。

摘　　要：探讨降香黄檀与桉树间作体系下根瘤与幼苗生长生理对不同浓度类黄酮处理的响应,为桉树人工林可持续经营提供理论依据。采用盆栽控制试验模拟尾巨桉-降香黄檀间作体系,外源金雀异黄酮、刺芒柄花素和大豆黄素3种类黄酮分别设置5μg·L^(-1)(N1)、10μg·L^(-1)(N2)和20μg·L^(-1)(N3)3个浓度梯度,以对照处理(CK)添加等量的无菌纯水。结果表明:3种类黄酮均能提高降香黄檀的结瘤能力,且在N3浓度的刺芒柄花素处理下,降香黄檀根瘤豆血红蛋白含量、单株瘤数和单株瘤质量优于其他处理;不同浓度类黄酮处理下,尾巨桉与降香黄檀的生长生理表现不一,类黄酮处理提高了间作体系下尾巨桉和降香黄檀植株的生理功能;冗余分析表明,降香黄檀的总根长、株高增量、叶生物量和过氧化物酶含量,尾巨桉的根生物量、株高增量和蒸腾速率与结瘤指标呈显著正相关;降香黄檀的丙二醛含量和尾巨桉的过氧化物酶、丙二醛含量和胞间二氧化碳浓度指标与结瘤指标呈显著负相关;相关性分析表明,降香黄檀结瘤能力与其叶胞间二氧化碳浓度、丙二醛含量呈显著负相关,与叶过氧化物酶活性呈显著正相关,根瘤固氮酶含量与尾巨桉叶胞间二氧化碳浓度呈显著负相关,单株瘤质量与尾巨桉叶丙二醛含量呈显著负相关。适量施加类黄酮物质对降香黄檀结瘤能力及两种树种幼苗的生长和生理均产生有利影响,且尾巨桉的生长发育及叶片生理活动在一定程度上与降香黄檀根部结瘤相关。Exploring the physiological responses of root nodules and seedling growth to different concentrations of flavonoids in the intercropping system of Dalbergiaodorifera and Eucalyptus,providing a theoretical basis for the sustainable management of Eucalyptus plantations.A pot experiment simulated the intercropping system of E.urophylla×E.grandis and D.odorifera using a completely randomized block design with two factors.The three flavonoids—exogenous genistein,manganese and daidzein—were applied at concentrations of 5μg•L^(-1)(N1),10μg•L^(-1)(N2)and 20μg•L^(-1)(N3),along with a control treatment(CK,using the same amount of sterile pure water).The results were as follows:All three flavonoids improved the nodulation ability of D.odorifera,with the highest hemoglobin content,nodule count,and weight per plant observed at the N3 concentration of spinocarpin compared to other treatments.The growth physiology of E.urophylla×E.grandis and D.odorifera differed under varying flavonoid concentrations,enhancing the physiological function of the E.urophylla×E.grandis and D.odorifera in intercropping system.Redundancy analysis showed that the total root length,plant height increment,leaf biomass,and peroxidase content of D.odorifera,as well as the root biomass,plant height increment,and transpiration rate of E.urophylla×E.grandis,were significantly positively correlated with nodulation indicators;the malondialdehyde content of D.odorifera and the peroxidase,malondialdehyde content,and intercellular carbon dioxide concentration indicators of E.urophylla×E.grandis were significantly negatively correlated with nodulation indicators.Redundancy analysis showed that the nodulation ability of D.odorifera was limited by its physiological status of D.odorifera and the biomass of E.urophylla×E.grandis.In conclusion,the judicious application of flavonoids positively influenced the nodulation ability of D.odorifera and the growth physiology of both tree species’seedings.The growth and development of E.urophylla×E.grandi

关 键 词：尾巨桉 降香黄檀 间作 类黄酮 

分 类 号：S718.43[农业科学—林学]