金耳代料栽培的基质配方优化  

Optimization of substrate formulation of Naematelia aurantialba substitute cultivation

作　　者：李宾 田芳 金丽 刘清超 LI Bin;TIAN Fang;JIN Li;LIU Qingchao(Zhengzhou Institute of Agricultural Science and Technology,Zhengzhou 450007,Henan,China;Taikang Shuirunjunzhao Agricultural Technology Co.,Ltd.,Taikang 461433,Henan,China)

机构地区：[1]郑州市农业科技研究院,郑州450007 [2]太康县水润君赵农业科技有限公司,河南太康461433

出　　处：《中国瓜菜》2025年第3期114-118,共5页China Cucurbits And Vegetables

基　　金：河南优势特色农业产业科技支撑行动计划(20240803002)。

摘　　要：以河南太康县栽培面积较大的J-1、J-2金耳菌株为研究对象,探索了7种不同的母种培养基对金耳J-2菌丝长势的影响,以及棉籽壳和木屑9种不同组成比例的栽培基质配方上J-1、J-2菌株的菌丝长势、出耳率及生物学效率。结果表明,金耳J-2菌株的母种最佳培养基为PDA培养基+3%麦粒煎汁,生长速率最大,为5.45 mm·d^(-1),菌丝长势最佳,菌丝生长指数最高,达到11 665.37,与其他6种培养基相比差异显著,玉米粉、蛋白胨的加入不利于金耳母种菌丝的生长。在本试验设计处理中,最佳栽培基质配方为:棉籽壳30%、木屑60%、蔗糖10%、玉米粉7.8%、生石膏1%、磷酸二氢钾0.2%,硫酸镁0.3%,J-1、J-2菌株100%出耳,生物学效率分别为69.31%、72.24%。试验结果为金耳栽培提供了科学的生产依据。The J-1 and J-2 Naematelia auriculus strains,which have a large cultivation area in Taikang county,Henan province,were used as research objects to explore the effects of 7 different culture media on the mycelia growth of J-2 Naematelia auriculus mother strain,as well as the mycelia growth,earing rate and biological efficiency of J-1 and J-2 strains on 9 different cultivation substrate formulae of cotton seed shell and wood chips.The results showed that the opti-mal mother culture media medium for J-2 strain was PDA medium with 3%wheat grain juice,which achieved the fastest growth rate of 5.45 mm·d^(-1),the best mycelial growth,and the highest mycelial growth index of 11665.37.The difference was significant compared with the other six media,and the addition of corn flour and peptone was not conducive to the mycelia growth of J-2 strain.In the experimental design,the optimal cultivation substrate formula was found to be 30%cotton seed shell,60%wood chips,10%sucrose,7.8%corn flour,1%gypsum,0.2%potassium dihydrogen phosphate,and 0.3%magnesium sulfate.With this formula,both J-1 and J-2 strains achieved a 100%earing rate,with the biological efficiency of 69.31%and 72.24%,respectively,providing a scientific basis for the cultivation of Naematelia auriculus.

关 键 词：金耳 母种培养基 菌丝长势 栽培基质 生物学效率 

分 类 号：S646.6[农业科学—蔬菜学]