大鼠周围神经损伤后前扣带回皮质Yes激酶相关蛋白表达变化  

Changes of Yes-associated protein expression in the anterior cingulate cortex after peripheral nerve injury in rats

作　　者：赵石祥 平苹 李治贵 黄美金 刘明沅 孔繁一 李娜 ZHAO Shi-xiang;PING Ping;LI Zhi-gui;HUANG Mei-jin;LIU Ming-yuan;KONG Fan-yi;LI Na(Department of Anesthesiology,920th Hospital of Joint Logistics Support Force,PLA,Kunming 650032,China;Graduate School of Kunming Medical University,Kunming 650500,China;Department of Oncology,920th Hospital of Joint Logistics Support Force,PLA,Kunming 650032,China;Department of Neurology,Affiliated Hospital of Yunnan University,Kunming 650021,China)

机构地区：[1]中国人民解放军联勤保障部队第九二〇医院麻醉科,昆明650032 [2]昆明医科大学研究生院,昆明650500 [3]中国人民解放军联勤保障部队第九二〇医院肿瘤科,昆明650032 [4]云南大学附属医院神经内科,昆明650021

出　　处：《中国疼痛医学杂志》2025年第3期172-180,共9页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金青年项目(81400924、81701117);云南省昆明医科大学联合专项应用基础研究计划面上项目(202201AY070001-284);云南省中青年学术和技术带头人后备人才项目(202305AC160069);昆明医科大学创新基金(2024S394)。

摘　　要：目的:探讨前扣带回皮质(anterior cingulate cortex,ACC)的Yes激酶相关蛋白(Yes-associated protein,YAP)在神经病理性疼痛(neuropathic pain,NP)中的表达变化。方法:建立坐骨神经慢性压迫损伤(chronic constriction injury of the sciatic nerve,CCI)大鼠模型,疼痛评估采用机械刺激缩足反射阈值(mechanical withdrawal threshold,MWT)和热缩足反射潜伏期(thermal withdrawal latency,TWL),通过蛋白质免疫印迹和免疫荧光检测YAP的表达变化,并观察YAP与神经元、星形胶质细胞和小胶质细胞共表达以及YAP的核定位情况。结果:与对照组比较,CCI组大鼠术后MWT和TWL显著降低,对侧ACC区域YAP蛋白表达显著升高,且YAP核表达量增加,YAP与神经元、星形胶质细胞和小胶质细胞存在共表达,CCI组YAP与神经元、星形胶质细胞的共定位数量显著增加。结论:外周神经损伤后大鼠ACC区域YAP核表达与蛋白表达增多,可能在NP的发生与维持中发挥重要作用。Objective:To investigate the changes of Yes-associated protein(YAP)in anterior cingulate cortex(ACC)in neuropathic pain(NP).Methods:Chronic constriction injury of the sciatic nerve(CCI)model was established.Rats were assessed pain behavior with mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL).Changes of YAP were detected by western blotting and immunofluorescence,and the co-expression of YAP with neurons,astrocytes,and microglia,as well as the nuclear localization of YAP were observed.Results:Compared with the Sham group,rats in the CCI group showed significantly reduced MWT and TWL after surgery,and a significant increase in YAP protein expression in the contralateral ACC region,with an increase in nuclear YAP expression.YAP co-expressed with neurons,astrocytes,and microglia in the ACC region of rats,and the number of YAP co-localizations with neurons and astrocytes significantly increased in the CCI group.Conclusion:The nuclear and protein expression of YAP in ACC area of rats increased following peripheral nerve injury may be a crucial role for the pathogenic process of neuropathic pain.

关 键 词：神经病理性疼痛 前扣带回皮质 Yes激酶相关蛋白 坐骨神经慢性压迫损伤 神经胶质细胞 

分 类 号：R745[医药卫生—神经病学与精神病学]